Changes in Main Biochemical Indices and Acetyl-CoA Carboxylase 1 mRNA Expression of Crass Carp Fed a High-Fat Diet

WANG Kaiyu; MIAO Changhong; HUANG Jinlu; WANG Jun; LIAN Hai; WU Chunyan; MOU Qiaofeng; FU Xi

doi:10.3969/j.issn.1006-267x.2012.12.012

Chinese Journal of Animal Nutrition >

2012 , Vol. 24 >Issue 12: 2375 - 2383

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.12.012

Fish Nutrition

Changes in Main Biochemical Indices and Acetyl-CoA Carboxylase 1 mRNA Expression of Crass Carp Fed a High-Fat Diet

WANG Kaiyu ,
MIAO Changhong ,
HUANG Jinlu ,
WANG Jun ,
LIAN Hai ,
WU Chunyan ,
MOU Qiaofeng ,
FU Xi

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Fisheries Disease Research Center, Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya’an 625014, China

Received date: 2012-07-24

Online published: 2012-12-03

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Abstract

The effects of consecutive feeding 8.1% fat diet for 12 weeks on serum biochemical indices, hepatopancreas biochemical indices and acetyl-CoA carboxylase 1 (ACC1) mRNA expression of grass carp were investigated to study the metabolic regulation mechanism of grass carp liver on high-fat diet. A total of 120 grass carp with an average body weight of (15.0±2.4) g were randomly divided into high-fat group and basal group, and the fish in those 2 groups were fed the diets containing 8.1% (high-fat diet) and 4.6% fat (basal diet), respectively. The contents of triglyceride (TG) and total cholesterol (CHO) and the activities of alanine aminotransferase (AST) and aspartate aminotransferase (ALT) in serum, and the content of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) and catalase (CAT) in hepatopancreas were determined on the 4th, 8th and 12th weeks, respectively. On the 12th week, the tissue morphology of hepatopancreas was observed by making pathological sections, and the relative expression of ACC1 mRNA in hepatopancreas was analyzed by semi-quantitative RT-PCR. The results showed as follows: in the experimental period, the hepatocytes of grass carp of high-fat group appeared injuries, and the injuries were more and more serious with increasing the feeding time; the contents of TG and CHO and the activities of AST and ALT in serum and the content of MDA in hepatopancreas of high-fat group were significantly increased (P＜0.05 or P＜0.01), while the activities of SOD and CAT in hepatopancreas of high-fat group were significantly decreased with increasing the feeding time (P＜0.05 or P＜0.01). On the 12th week, the contents of TG and CHO and the activities of AST and ALT in serum and the content of MDA in hepatopancreas of high-fat group were significantly higher than those of basal group (P<0.05 or P＜0.01), whereas the activities of SOD and CAT in hepatopancreas of high-fat group were significantly lower than those of basal group (P<0.05 or P＜0.01). Compared with the basal group, the relative expression of ACC1 mRNA of high-fat group was significantly increased on the 12th week (P<0.01). The results indicate that consecutive feeding high-fat diet can increase the blood fat level and damage the hepatopancreas of grass carp, and its mechanism may be related to the high-fat diet decreasing antioxidant ability and increasing liver fat synthesis of grass carp.

Key words： grass carp; high-fat diet; biochemical indices; ACC1 mRNA; semi-quantitative RT-PCR

Cite this article

WANG Kaiyu , MIAO Changhong , HUANG Jinlu , WANG Jun , LIAN Hai , WU Chunyan , MOU Qiaofeng , FU Xi . Changes in Main Biochemical Indices and Acetyl-CoA Carboxylase 1 mRNA Expression of Crass Carp Fed a High-Fat Diet[J]. Chinese Journal of Animal Nutrition, 2012 , 24(12) : 2375 -2383 . DOI: 10.3969/j.issn.1006-267x.2012.12.012

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