Glutaredoxin 1 and Thioredoxin 1: Gene Expression Characteristics in Different Tissues and Effects of L-Histidine on Gene Expressions in Oxidant Stress Cells of Yunnan Wujin Pigs

ZHANG Chunyong; CHEN Kelin; HUANG Jinchang; GUO Rongfu

doi:10.3969/j.issn.1006-267x.2012.12.016

2012 , Vol. 24 >Issue 12: 2415 - 2423

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.12.016

Molecular Nutrition

Glutaredoxin 1 and Thioredoxin 1: Gene Expression Characteristics in Different Tissues and Effects of L-Histidine on Gene Expressions in Oxidant Stress Cells of Yunnan Wujin Pigs

ZHANG Chunyong ,
CHEN Kelin ,
HUANG Jinchang ,
GUO Rongfu

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Yunnan Agricultural University, Yunnan Key Laboratory of Animal Nutrition and Feeds, Kunming 650201, China

Received date: 2012-06-24

Online published: 2012-12-03

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Abstract

This experiment was conducted to investigate the gene expressions of glutaredoxin 1 (GRX1) and thioredoxin 1 (TRX1) in pituitary, hypothalamus, thyroid, thymus, pancreas, gonad, liver, skin, duodenum, jejunum and ileum tissues and explore the regulation of L-histidine on gene expressions in oxidative stress cells of Yunnan Wujin pigs. Different expressions of GRX1 and TRX1 genes were determined by the method of real-time quantitative PCR (RT-qPCR), and the regulation of L-histidine on GRX1 and TRX1 gene expressions was explored in oxidative stress cells induced by H₂O₂. The results showed as follows: 1) GRX1 and TRX1 gene expressions in tissues of Wujin pigs were detected, the highest gene expressions of GRX1 and TRX1 were in the liver, and then skin and jejunum, and relatively lower gene expressions were in the other tissues. 2) TRX1 gene expression in tissues was higher than GRX1 gene expression for Wujin pigs. 3) The results of cell culture showed that when being induced by H₂O₂, oxidative stress cells produced the over expression of GRX1 and TRX1 genes. L-histidine had the regulation on GRX1 and TRX1 gene expressions in oxidative stress or no stress cells, being the adequate concentration of 280 μg/mL. GRX1 and TRX1 gene expressions had the obvious tissue characteristics for Wujin pigs. H₂O₂ could induce the over expression of GRX1 and TRX1 genes in oxidative stress cells. Adding adequate concentration of L-histidine could regulate GRX1 and TRX1 gene expressions in oxidative stress or no stress cells of Wujin pigs. It is suggested that this may be an important way of reducing the oxidative stress damage and improving the oxidation resistance, being GRX1 and TRX1 gene expressions of animal body induced by nutritional pathway for Wujin pigs.

Key words： Wujin pigs; oxidative stress; GRX1; TRX1; gene expression; L-histidine

Cite this article

ZHANG Chunyong , CHEN Kelin , HUANG Jinchang , GUO Rongfu . Glutaredoxin 1 and Thioredoxin 1: Gene Expression Characteristics in Different Tissues and Effects of L-Histidine on Gene Expressions in Oxidant Stress Cells of Yunnan Wujin Pigs[J]. Chinese Journal of Animal Nutrition, 2012 , 24(12) : 2415 -2423 . DOI: 10.3969/j.issn.1006-267x.2012.12.016

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