A Comparative Study of Fasting Method and Nitrogen-Free Diet Method for Determining Endougenous Amino Acid Excretion in Yellow-Feathered Chickens

REN Liqin; ZHAO Feng; TAN Huize; ZHANG Jianzhi; MI Baomin; ZHAO Jiangtao; ZHANG Hongfu

doi:10.3969/j.issn.1006-267x.2012.12.017

2012 , Vol. 24 >Issue 12: 2424 - 2435

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.12.017

Experimental Methods

A Comparative Study of Fasting Method and Nitrogen-Free Diet Method for Determining Endougenous Amino Acid Excretion in Yellow-Feathered Chickens

REN Liqin ,
ZHAO Feng ,
TAN Huize ,
ZHANG Jianzhi ,
MI Baomin ,
ZHAO Jiangtao ,
ZHANG Hongfu

Expand

1. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. Wen’s Foodstuffs Group Corporation Co. , Ltd. , Guangzhou 527439, China

Received date: 2012-06-01

Online published: 2012-12-03

Fold

Abstract

This experiment was conducted to compare the excretion and variation of endogenous amino acids (EAA) in yellow-feathered chickens under fasting and nitrogen-free diet (NFD) to evaluate the methods for the EAA determination in the measurement of the digestible amino acids of ingredients. Using a single factor completely randomized design, a total of 72 adult roosters aged 15 weeks were randomly selected according to their initial body weight and divided into 3 groups with 6 replicates per group and 4 roosters per replicate. The same experiment was repeated 4 batches to determine the EAA excretion under fasting, 25 g NFD, and 40 g NFD, respectively. The results showed as follows: the EAA excretion was increased significantly from the groups of fasting, 25 g NFD to 40 g NFD (P<0.05). In the fasting group, the total coefficient of variation (CV_total), intragroup coefficient of variation (CV_within), and intergroup coefficient of variation (CV_between) of the EAA excretion of 17 amino acids among among 4 batches were from 9.74% to 19.99%, from 9.97% to 16.88%, and from 2.58% to 16.23%, respectively. The differences of EAA excretion of threonine,cystine,serine and proline among 4 batches were significant (P<0.05). In the 25 g NFD group, the CV_total, CV_within, and CV_between of the EAA excretion of 17 amino acids among 4 batches were from 17.77% to 31.33%, from 14.99% to 28.80%, and from 3.89% to 17.04%, respectively. The differences of EAA excretion of arginine, glycine and proline among 4 batches were significant (P<0.05). In the 40 g NFD group, the CV_total, CV_within, and CV_between of the EAA excretion of 17 amino acids among 4 batches were from 16.23% to 33.39%, from 17.15% to 33.82%, and from 4.19% to 16.29%, respectively. The differences of EAA excretion of isoleucine and phenylalanine among 4 batches were significant (P<0.05). It can be concluded that the excretion of EAA increases with the increasing amount of force-fed diet. The variation coefficients of the 25 and 40 g force-fed group are similar, and are both larger than those of fasting group.

Key words： endogenous amino acids; fasting method; nitrogen-free diet method; yellow-feathered chicken

Cite this article

REN Liqin , ZHAO Feng , TAN Huize , ZHANG Jianzhi , MI Baomin , ZHAO Jiangtao , ZHANG Hongfu . A Comparative Study of Fasting Method and Nitrogen-Free Diet Method for Determining Endougenous Amino Acid Excretion in Yellow-Feathered Chickens[J]. Chinese Journal of Animal Nutrition, 2012 , 24(12) : 2424 -2435 . DOI: 10.3969/j.issn.1006-267x.2012.12.017

References

[1] SIBBALD I R.A bioassay for available amino acids and true metabolizable energy in feeding stuffs[J].Poultry Science,1979,58:668-673.
[2] SONG G L,LI D F,PIAO X S,et al.Comparisons of amino acid availability by different methods and metabolizable energy determination of a Chinese variety of high oil corn[J].Poultry Science,2003,82:1017-1023.
[3] KLUTH H,RODEHUTSCORD M.Effect of inclusion of cellulose in the diet on the inevitable endogenous amino acid losses in the ileum of broiler chickens[J].Poultry Science,2009,88:1199-1205.
[4] SIBBALD I R.A bioassay for true metabolizable energy in feedstuffs[J].Poultry Science,1976,55:303-308.
[5] MCNAB J M,FISHER C.The choice between apparent and true metabolizable energy systems-recent evidence //Proceedings of the 3rd European symposium on poultry nutrition.Edinburgh: ,1981:45-55.
[6] ENGSTER H M,CAVE N A,LIKUSKI H,et al.A collaborative study to evaluate a precision-fed rooster assay for true amino acid availability in feed ingredients[J].Poultry Science,1985,64:487-498.
[7] 黎观红,瞿明仁,朱年华,等.泰和鸡内源氨基酸排泄量的研究[J].动物营养学报,2002,4(1):42-44.
[8] 翟少伟,曹平华.两种常用方法测定鸡内源氨基酸损失量的比较研究[J].西北农业学报,2003,12(2):10-13.
[9] MUZTAR A J,SLINGER S J.Effect of level of dietary fiber on nitrogen and amino acid excretion in the fasted mature rooster[J].Nutrition Reports International,1980,22:863-868.
[10] LIKUSKI H J A,DORRELL H G.A bioassay for rapid determination of amino acid availability values[J].Poultry Science,1978,57:1658-1660.
[11] 全国饲料工业标准化技术委员会.GB/T 26437—2010畜禽饲料有效性与安全性评价——强饲法测定鸡饲料表观代谢能技术规程[S].北京:中国标准出版社,2010.
[12] REN L Q,TAN H Z,ZHAO F,et al.Applicability of corn starch as basal diet in determining the true metabolizable energy of protein feedstuffs in Chinese yellow chickens[J].Poultry Science,2012,91:1394-1399.
[13] CHUNG T K,BAKER D H.Apparent and true amino acid digestibility of a crystalline amino acid mixture and of casein:comparison of values obtained with ileal-cannulated pigs and cecectomized cockerels[J].Journal of Animal Science,1992,70:3781-3790.
[14] PARSONS C M,POTTER L M,BROWN R D.Effects of dietary carbohydrate and of intestinal microflora on excretion of endogenous amino acids by poultry[J].Poultry Science,1983,62:483-489.
[15] ADEDOKUN S A,LILBURN M S,PARSONS C M,et al.Endogenous amino acid flow in broiler chicks is affected by the age of birds and method of estimation[J].Poultry Science,2007,86:2590-2597.
[16] BOURDILLON A,CARRE B,CONAN L,et al.European reference method for the in vivo determination of metabolisable energy with adult cockerels:reproducibility,effect of food intake and comparison with individual laboratory methods[J].British Poultry Science,1990,31(3):557-565.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References