Phosphorus in Vitro Dialyzability of Several Common Plant-Origin Feed Ingredients for Pigs Evaluated by Bionic Digestion System

ZHUANG Xiaofeng; CHEN Liang; ZHANG Hongfu; ZHAO Feng; LU Qingping; SAREN Na; LI Dongwei; HE Kelin

doi:10.3969/j.issn.1006-267x.2012.12.018

2012 , Vol. 24 >Issue 12: 2436 - 2443

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.12.018

Experimental Methods

Phosphorus in Vitro Dialyzability of Several Common Plant-Origin Feed Ingredients for Pigs Evaluated by Bionic Digestion System

ZHUANG Xiaofeng ,
CHEN Liang ,
ZHANG Hongfu ,
ZHAO Feng ,
LU Qingping ,
SAREN Na ,
LI Dongwei ,
HE Kelin

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State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2012-06-11

Online published: 2012-12-03

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Abstract

The total phosphorus dialyzability and inorganic phosphorus dialyzability of several feed ingredients determined using different molecular weight cutoff (MWCO) dialysis tubings (DT) were investigated for testing whether bionic digestive system could be used for determining phosphorus in vitro dialyzability. The total phosphorus in undigested residue and inorganic phosphorus in dialysate was determined in experiment 1, which was to study whether MWCO 14 000 or 3 500 u DT could separate inorganic phosphorus and organic phosphorus. In experiment 2, the phosphorus in vitro dialyzability of swine common feed ingredients (corn, soybean meal, rice bran, rapeseed meal and wheat bran) was measured by determining inorganic phosphorus in dialysate using MWCO 14 000 or 3 500 u DT, which was to study the feasibility of using inorganic phosphorus determination to measure phosphorus in vitro dialyzability. The results showed as follows: 1) there was organic phosphorus in dialysate, which indicated that MWCO 3 500 u DT was unable to separate inorganic phosphorus and organic phosphorus. 2) In inorganic phosphorus determination experiment, the phosphorus in vitro dialyzability determined using MWCO 14 000 u DT was significantly higher than that determined using 3 500 u DT (P<0.05). 3) The coefficients of variation of the phosphorus dialyzability in the experiment of inorganic phosphorus determination were all less than 4%, which was lower than the results reported by other researchers. In conclusion, MWCO 3 500 u DT is unable to separate inorganic phosphorus and organic phosphorus, so the phosphorus in vitro dialyzability can not be measured by determining the total phosphorus in undigested residue; the results of inorganic phosphorus determination using MWCO 14 000 u DT is higher and accurate, so the method is more appropriate for measuring phosphorus in vitro dialyzability of feed ingredients for pigs.

Key words： feed ingredients for pigs; dialysis tubing; phosphorus in vitro dialyzability; inorganic phosphorus determination; bionic digestive system

Cite this article

ZHUANG Xiaofeng , CHEN Liang , ZHANG Hongfu , ZHAO Feng , LU Qingping , SAREN Na , LI Dongwei , HE Kelin . Phosphorus in Vitro Dialyzability of Several Common Plant-Origin Feed Ingredients for Pigs Evaluated by Bionic Digestion System[J]. Chinese Journal of Animal Nutrition, 2012 , 24(12) : 2436 -2443 . DOI: 10.3969/j.issn.1006-267x.2012.12.018

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