This study was conducted to investigate the effects of asparagine (ASN) on growth performance, blood cell differential count and blood biochemical indices of weaner pigs challenged with lipopolysaccharide (LPS). Twenty-four weaner piglets (Duroc×Landrace×Large) were randomly allocated into 4 groups with 6 replicates and 1 piglet per replicate. The four groups included control group (basal diet), LPS group (basal diet+LPS), 0.5%ASN group (basal diet+LPS+0.5%ASN) and 1.0%ASN group (basal diet+LPS+1.0%ASN). On day 16, the piglets in the LPS group, 0.5%ASN group and 1.0%ASN group were injected intraperitoneally with 100 μg/kg BW LPS, and the piglets in the control group were injected intraperitoneally with the same dose of physiological saline. The results showed as follows: 1) 0.5%ASN group significantly alleviated the decrease of average daily gain (ADG) induced by LPS (P<0.05). 2) At 4 h, 0.5%ASN group significantly increased the percentage of lymphocytes and monocytes (P<0.05), and 1.0%ASN group significantly alleviated the increase of eosinophilic granulocyte percentage and the decrease of platelet number induced by LPS (P<0.05). At 24 h, 0.5%ASN alleviated the decrease of red blood cell number and hematokrit induced by LPS (P<0.05). 1.0%ASN group significantly alleviated the increase of the number of white blood cell and neutrophili induced by LPS (P<0.05), also significantly alleviated the decrease of lymphocyte number and percentage, hemoglobin content and hematocrit induced by LPS (P<0.05). 3) At 24 h, 0.5%ASN group significantly alleviated the increase of glutamic oxalacetic transaminase (GOT) activity and the decrease of blood glucose content induced by LPS (P<0.05). 1.0%ASN group significantly alleviated the increase of GOT and the decrease of glutamic-pyruvic transaminase (GPT)/GOT value induced by LPS (P<0.05), significantly increased the total protein content, and decreased the alkaline phosphatase activity (P<0.05). In conclusion, ASN can alleviate the growth depression and stress response induced by LPS to some degree.
LI Shuang
,
LIU Yulan
,
SHI Haifeng
,
CHENG Feng
,
ZHU Huiling
,
HOU Yongqing
,
DING Binying
,
PI Dingan
,
LENG Weibo
. Effects of Asparagine on Growth Performance, Blood Cell Differential Count and Blood Biochemical Indices of Weaner Pigs Challenged with Lipopolysaccharide[J]. Chinese Journal of Animal Nutrition, 2012
, 24(12)
: 2450
-2458
.
DOI: 10.3969/j.issn.1006-267x.2012.12.020
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