Inhibitors of Mitogen Activated Protein Kinase Pathways: Effects on Intestinal Morphology and Permeability of Weaner Piglets

LUAN Zhaoshuang; SONG Juan; HU Caihong

doi:10.3969/j.issn.1006-267x.2013.01.007

2013 , Vol. 25 >Issue 1: 44 - 49

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.01.007

Swine Nutrition

Inhibitors of Mitogen Activated Protein Kinase Pathways: Effects on Intestinal Morphology and Permeability of Weaner Piglets

LUAN Zhaoshuang ,
SONG Juan ,
HU Caihong

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The Key Laboratory of Molecular Animal Nutrition, Institute of Feed Science, Zhejiang University, Hangzhou 310058, China

Received date: 2012-07-02

Online published: 2013-01-04

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Abstract

This experiment was conducted to investigate the effects of inhibitors of mitogen activated protein kinase pathways on intestinal morphology and permeability of weaner piglets. Twenty-four weaner piglets (Duroc×Landrace×Yorkshire) with a similar body weight of 5.8 kg were randomly allocated to 4 groups with 6 piglets in each group. Thirty minutes before weaning, piglets in experimental groups received intraperitoneal injection of inhibitors of p38 MAPK (SB203580, group Ⅰ), JNK (SP600125, group Ⅱ) and ERK1/2 (PD98059, group Ⅲ), respectively. Piglets in control group were given the same volume of saline. Piglets were slaughtered at 36 h after weaning. The results showed as follows: compared with control group, group Ⅰ had significantly higher jejunum villus height and villus height/crypt depth (P<0.05), and group Ⅱ had significantly higher villus height/crypt depth (P<0.05); compared with control group, contents of plasma D-lactate and DAO in groups Ⅰ and Ⅱ were significantly decreased (P<0.05), while plasma D-lactate content in group Ⅲ was significantly increased (P<0.05); compared with the control group, levels of proinflammatory cytokines (TNF-α, IL-1β, IL-6 and IFN-γ) in group Ⅰ were significantly decreased (P<0.05), TNF-α and IL-1β levels in group Ⅱ were significantly decreased (P<0.05), however, TNF-α level in group Ⅲ was significantly increased (P<0.05), IL-6 and IFN-γ levels tended to be increased (P>0.05). In conclusion, inhibition of p38 MAPK and JNK pathways protects intestinal mucosal barrier from the damage induced by early weaning. However, there are worse damage in intestinal mucosal barriers when ERK1/2 signaling pathway is inhibited.

Key words： weaner piglets; intestinal morphology; intestinal permeability; MAPK signaling pathways

Cite this article

LUAN Zhaoshuang , SONG Juan , HU Caihong . Inhibitors of Mitogen Activated Protein Kinase Pathways: Effects on Intestinal Morphology and Permeability of Weaner Piglets[J]. Chinese Journal of Animal Nutrition, 2013 , 25(1) : 44 -49 . DOI: 10.3969/j.issn.1006-267x.2013.01.007

References

[1] 郑曙云,付小兵,徐建国.MAPK信号传导通路与肠损伤后黏膜上皮修复[J].中国危重病急救医学,2004,16(1):59-62.
[2] BROOM O J,WIDJAYA B,TROELSEN J,et al.Mitogen activated protein kinases:a role in inflammatory bowel disease?[J].Clinical & Experimental Immunology,2009,158(3):272-280.

[3] COSTANTINI T W,PETERSON C Y,KROLL L,et al.Role of p38 MAPK in burn-induced intestinal barrier breakdown[J].Journal of Surgical Research,2009,156(1):64-69.

[4] LALLS J P,BOSI P,SMIDT H,et al.Weaning:a challenge to gut physiologists[J].Livestock Science,2007,108(1/2/3):82-93.
[5] 刘海萍,胡彩虹,徐勇.早期断奶对仔猪肠通透性和肠上皮紧密连接蛋白Occludin mRNA表达的影响[J].动物营养学报,2008,20(4):442-446.
[6] HU C H,SONG J,YOU Z T,et al.Zinc oxide-montmorillonite hybrid influences diarrhea,intestinal mucosal integrity and digestive enzyme activity in weaned pigs[J].Biological Trace Element Research,2011.doi:10.1007/s12011-012-9422-9.
[7] MOESER A J,VANDER K C,RYAN K A,et al.Stress signaling pathways activated by weaning mediate intestinal dysfunction in the pig[J].American Journal of Physiology:Gastrointestinal and Liver Physiology,2007,292(1):G173-G181.
[8] BRANDT R B,SIEGEL S A,WATERS M G,et al.Spectrophotometric assay for D-lactate in plasma[J].Analytical Biochemistry,1980,102(1):39-46.

[9] 胡泉舟,侯永清,王猛.血中二胺氧化酶活性与仔猪腹泻程度的相关性分析[J].猪业科学,2007(12):73-74.
[10] KYRIAKIS J M,BANERJEE P,NIKOLAKAKI E,et al.The stress-activated protein kinase subfamily of c-Jun kinases[J].Nature,1994,369:156-160.
[11] HEDEMANN M S,DYBKJAER L,JENSEN B B.Pre-weaning eating activity and morphological parameters in the small and large intestine of piglets[J].Livestock Science,2007,108(1/2/3):128-131.
[12] HEDEMANN M S,HJSGAARD S,JENSEN B B.Small intestinal morphology and activity of intestinal peptidases in piglets around weaning[J].Journal of Animal Physiology and Animal Nutrition,2003,87(1/2):32-41.
[13] WIJTTEN P J A,MEULEN J V D,VERSTEGEN M W A.Intestinal barrier function and absorption in pigs after weaning:a review[J].British Journal of Nutrition,2011,105:967-981.
[14] SMITH F,CLARK J E,OVERMAN B L,et al.Early weaning stress impairs development of mucosal barrier function in the porcine intestine[J].American Journal of Physiology:Gastrointestinal and Liver Physiology,2010,298:G352-G363.
[15] 栾兆双,姚丽丽,傅振宁,等.断奶应激对仔猪肠形态、肠黏膜屏障和p38丝裂原活化蛋白激酶信号通路的影响[J].动物营养学报,2012,24(11):2237-2242.
[16] PI S,LALLS J P,BLAZY F,et al.Weaning is associated with an upregulation of expression of inflammatory cytokines in the intestine of piglets[J].The Journal of Nutrition,2004,134:641-647.
[17] 易文全,甘华田.P38 MAPK信号通路:炎性肠病治疗的新靶[J].华西医学,2006,21(1):194-195.
[18] MIHAESCU A,SANTEN S,JEPPSSON B,et al.P38 mitogen-activated protein kinase signalling regulates vascular inflammation and epithelial barrier dysfunction in an experimental model of radiation-induced colitis[J].British Journal of Surgery,2010,97(2):226-234.

[19] CAPALDO C T,NUSRAT A.Cytokine regulation of tight junctions[J].Biochimica et Biophysica Acta,2009,1788(4):864-871.

[20] BASUROY S,SETH A,ELIAS B,et al.MAPK interacts with occludin and mediates EGF-induced prevention of tight junction disruption by hydrogen peroxide[J].Biochemical Journal,2006,(393):69-77.
[21] 余进.猪和大鼠小肠黏膜热应激损伤修复机制的研究 .硕士学位论文.北京:北京农学院,2010:57-69.

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