Effects of Insulin on Growth and Expressions of &kappa;-Casein and Insulin Receptor Genes in Bovine Mammary Epithelial Cells

TIAN Qing; JI Yun; PANG Xueyan; WANG Hongrong

doi:10.3969/j.issn.1006-267x.2013.01.011

Chinese Journal of Animal Nutrition >

2013 , Vol. 25 >Issue 1: 77 - 87

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.01.011

Ruminant Nutrition

Effects of Insulin on Growth and Expressions of κ-Casein and Insulin Receptor Genes in Bovine Mammary Epithelial Cells

TIAN Qing ,
JI Yun ,
PANG Xueyan ,
WANG Hongrong

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College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2012-07-11

Online published: 2013-01-04

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Abstract

This study was conducted to investigate the effects of insulin on growth and expressions of κ-casein (CSN3) and insulin receptor (INSR) genes in bovine mammary epithelial cells. Bovine mammary epithelial cells from Chinese Holstein were used in an in vitro cultivation. Cells were cultured in a growth culture medium (without serum and hormone, control group), and the growth culture medium supplemented with 5, 50, 500 and 5 000 ng/mL insulin, respectively. Changes of expression levels of CSN3 and INSR genes and relative content of CSN3 were determined. The results showed as follows: 1) insulin could promote the proliferation of bovine mammary epithelial cells, and the optimal level was 5 to 500 ng/mL. 2) The expression levels of CSN3 and INSR genes and relative content of CSN3 were increased by insulin, CSN3 gene expression level and CSN3 relative content in 50 ng/mL group were significantly higher than those in control group (P<0.01) as well as 5(P<0.01), 500(P<0.01) and 5 000 ng/mL groups (P<0.05), and INSR gene expression level was significantly higher than that in control group (P<0.05) as well as 500 (P<0.05) and 5 000 ng/mL groups (P<0.01). The results indicate that with the increase of insulin concentration, expression levels of CSN3 and INSR genes and relative content of CSN3 tend to be increased at first and then decreased, and the peak appears at 50 ng/mL, while high concentration of insulin (>5 000 ng/mL) is unfavourable for the synthesis of milk protein in bovine mammary epithelial cells.

Key words： insulin; insulin receptor; κ-casein; bovine mammary epithelial cell; gene expression; cell proliferation

Cite this article

TIAN Qing , JI Yun , PANG Xueyan , WANG Hongrong . Effects of Insulin on Growth and Expressions of κ-Casein and Insulin Receptor Genes in Bovine Mammary Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2013 , 25(1) : 77 -87 . DOI: 10.3969/j.issn.1006-267x.2013.01.011

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