This study was to investigate the effects of supplementation of selenium and vitamin E on rumen fermentation and urinary purine derivative contents in Simmental steers. Four Simmental steers [average body weight of (500?20) kg, 5 years old] with permanent rumen fistulas were used in a 4?4 two-factor design. Those steers were fed a basal diet consisted of corn silage and concentrate supplement which was added with 0, 0.1, 0.2 and 0.3 mg/kg selenium and 0, 30, 60 and 90 IU/kg vitamin E. Selenium (mg/kg) /vitamin E (IU/kg) levels in the sixteen groups were 0/0 (group 1), 0.1/0 (group 2), 0.2/0 (group 3), 0.3/0 (group 4), 0/30 (group 5), 0.1/30 (group 6), 0.2/30 (group 7), 0.3/30 (group 8), 0/60 (group 9), 0.1/60 (group 10), 0.2/60 (group 11), 0.3/60 (group 12), 0/90 (group 13), 0.1/90 (group 14), 0.2/90 (group 15) and 0.3/90 (group 16), respectively. The results showed as follows: 1) ruminal pH in groups 4, 8 and 12 was significantly lower than that in groups 1, 5, 9 and 13 (P<0.05). Ammonia nitrogen content was significantly different between groups 1 to 4 and groups 9 to 16, between groups 5 to 8 and groups 12 to 16 (P<0.01), respectively, and that in groups 5 to 8 was significantly higher than that in groups 9 to 11 (P<0.05). The contents of acetate, propionate and butyrate in groups 8 and 12 were significantly higher than those in group 1 (P<0.05), and the total volatile fatty acid content in groups 8, 12 and 14 was significantly higher than that in groups 5, 9 and 13 (P<0.05). 2) Organic matter degradability of corn straw in groups 4, 8, 12 and 14 was significantly higher than that in groups 1, 2 and 3 (P<0.01), and significantly higher than that in groups 5, 6, 9 and 13 (P<0.05). Neutral detergent fiber degradability of corn straw in groups 4, 8, 12 and 16 was significantly higher than that in groups 1, 3, 5, 9 and 13 (P<0.01). Acid detergent fiber degradability of corn straw in groups 8 and 12 was significantly higher than that in groups 1, 3, 5, 9, 11, 13, 14 and 15 (P<0.01). 3) Organic matter degradability of concentrate supplement in group 14 was significantly higher than that in group 1 (P<0.05); crude protein degradability in group 4 was significantly higher than that in group 10 (P<0.05). 4) The contents of allantoin and uric acid in groups 8, 12 and 14 were significantly higher than those in group 1 (P<0.05); purine derivative content in group 8 was significantly higher than that in group 1 (P<0.01), and that in groups 12 and 14 was significantly higher than that in group 1 (P<0.05). The results indicate that selenium and vitamin E has a positive influence on rumen fermentation and urinary purine derivative contents. The optimum selenium and vitamin E levels are 0.3 mg/kg and 30 IU/kg, respectively.
ZHANG Shuanlin
,
YUAN Xia
,
XU Yaguang
,
CHENG Jianguo
,
WU Jinxiao
,
NIE Yumei
,
HUANG Yingxiang
,
YANG Zhiling
. Effects of Selenium and Vitamin E on Rumen Fermentation and Urinary Purine Derivative Contents in Simmental Steers[J]. Chinese Journal of Animal Nutrition, 2013
, 25(2)
: 326
-333
.
DOI: 10.3969/j.issn.1006-267x.2013.02.013
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