The objective of this study was to investigate the effects of alfalfa meal, soybean meal, maize and concentrate mixture replaced by big leaf mulberry meal on rumen in vitro fermentation characteristics, and to obtain the optimal replacement ratio. The rumen fluid was collected from four castrated young Hu sheep fitted with permanent rumen fistulas. The study contained 4 experiments, the big leaf mulberry meal was used to replace 100% alfalfa meal, 25% to 100% soybean meal and 10% to 40% maize or concentrate mixture, respectively, and every experiment had a control. In vitro gas production test was performed to determine the rumen fermentation parameters in 24 h. The results showed as follows: compared with the control, no significant differences were observed in the pH, gas production in 24 h and ammonia-N concentration when 100% alfalfa meal was replaced by big leaf mulberry meal (P>0.05), but the concentration of total volatile fatty acids (TVFA) was significantly decreased (P<0.05), and the ratios of volatile fatty acids (VFA) to TVFA were not significantly different (P>0.05). The gas production in 24 h and rumen fermentation parameters did not change when 50% soybean meal was replaced by big leaf mulberry meal (P>0.05), while the higher replacement ratio significantly reduced gas production in 24 h, pH and ammonia-N concentration (P<0.05). With the increase of replacement ratio of big leaf mulberry meal to maize, gas production in 24 h did not change (P>0.05), but the pH and ammonia-N concentration were significantly increased (P<0.05), while TVFA concentration was significantly decreased (P<0.05), acetate/TVFA was significantly increased (P<0.05), and propionate/TVFA was decreased, but was not significantly different (P>0.05). No statistical differences were observed in pH and ammonia-N concentration when concentrate mixture was replaced by big leaf mulberry meal (P>0.05). The replacement ratio less than 20% did not change the gas production in 24 h and fermentation parameters (except acetate /propionate) (P>0.05), but the replacement ratio higher than 40% significantly reduced gas production in 24 h and TVFA concentration (P<0.05). From the above results, it is inferred that there is no significant difference in rumen in vitro fermentation characteristics when 100% alfalfa meal is replaced by big leaf mulberry meal, and the influences on rumen fermentation parameters are not significant when 50% soybean meal is replaced by big leaf mulberry meal, while replacement ratio of maize or concentrate mixture should not exceed 20%.
MA Meirong
,
LIU Jianxin
,
YANG Fang
. Effects of Alfalfa Meal, Soybean Meal, Maize and Concentrate Mixture Replaced by Big Leaf Mulberry Meal on Rumen in Vitro Fermentation Characteristics[J]. Chinese Journal of Animal Nutrition, 2013
, 25(2)
: 397
-405
.
DOI: 10.3969/j.issn.1006-267x.2013.02.022
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