The aims of this experiment were to optimize the extraction technology and to study the antioxidant activity of Castanea mollissima Blume polyphenols. The orthogonal test was adopted and the factors influencing the process parameters were determined. The antioxidant activity was evaluated through detecting DPPH· and ABTS+· scavenging rates, and peroxide value (POV) of Castanea mollissima Blume polyphenols in vitro. The results showed as follows: 1) optimal conditions for the extraction: ethanol concentration was 30%, liquid-solid ratio was 18:1 (mL:g), extraction temperature was 80 ℃, and extraction time was 140 min. The corresponding extraction yield was 22.61%, and the polyphenol content was 51.23%. 2) The DPPH· and ABTS+· scavenging rates of polyphenols from Castanea mollissima Blume were similar to that of vitamin C, and higher than that of vitamin E. Castanea mollissima Blume polyphenols displayed an excellent antioxidant activity in the lard oil and the effect depended on its concentration, with the best concentration of 100 μg/mg. The antioxidant effect of Castanea mollissima Blume polyphenols at this concentration was equal to that of vitamin C, and both of them were better than vitamin E. In conclusion, under the optimal conditions, Castanea mollissima Blume polyphenols shows higher extraction yield and content and reveals better antioxidant activity.
SHI Enhui
,
GUO Kaijun
,
LI Hong
,
GU Mingcan
,
LU Lin
,
JIA Changxi
. Castanea mollissima Blume Polyphenols: Extraction Technology Optimization and Its Antioxidant Activity[J]. Chinese Journal of Animal Nutrition, 2013
, 25(2)
: 406
-414
.
DOI: 10.3969/j.issn.1006-267x.2013.02.023
[1] 石碧,狄莹.植物多酚[M].北京:科学出版社,2000:1-2.
[2] OKUDA T,KIMURA Y,YOSHIDA T,et al.Studies on activities of tannins and related compounds from medicinal plants and drugs.Ⅰ.Inhibitory effects on lipid peroxidation in mitochondria and microsomes of liver[J].Chemical & Pharmaceutical Bulletin,1983,31(5):1625-1631.
[3] KIMURA Y,OKUDA H,MORI K,et al.Studies on the activities of tannins and related compounds from medicinal plants and drugs.Ⅳ.Effects of various extracts of Geranii herba and geraniin on liver injury and lipid metabolism in rats fed peroxidatal oil[J].Chemical & Pharmaceutical Bulletin,1984,32(5):1866-1871.
[4] TAKECHI M,TANKA Y,TAKEHARA M,et al.Structure and antiherpetic activity among the tannins[J].Phytochemistry,1985,24(10):2245-2250.
[5] GALI U,PERCHELLET E,KLISK D,et al.Antitumor-promoting activities of hydrolyzable tannins in mouse skin[J].Carcinogensis,1992,13(14):715-718.
[6] YOSHIDA T,TONG C,MATSUDA M,et al.Woodfordin D and oenothein A,trimeric hydrolyzable tannins of marco-ring structure with antitumor activity[J].Chemical & Pharmaceutical Bulletin,1991,39(5):1157-1162.
[7] BARREIRA J C M,FERREIRA I C F R,OLIVEIRA M B P P,et al.Antioxidant activities of the extracts from chestnut flower,leaf,skins and fruit[J].Food Chemistry,107(3):1106-1113.
[8] TABACCO E,BORREANI G,CROVETTO G M,et al.Effect of chestnut tannin on fermentation quality,proteolysis and protein rumen degradability of alfalfa silage[J].Journal of Dairy Science,2006,89:4736-4746.
[9] SCHIAVONE A,GUO K,TASSONE S,et al.Effects of a natural extract of chestnut wood on digestibility,performance traits,and nitrogen balance of broiler chicks[J].Poultry Science,2008,87:521-527.
[10] ZOCCARATO I,GASCO L,SCHIAVONE A,et al.Effect of extract of chestnut wood inclusion (ENC ) in normal and low protein amino acid supplemented diets on heavy broiler rabbits //Proceedings of the 9th world rabbit congress.Verona: ,2008:873-877.
[11] LIU H W,XIAO F D,JIAN M T,et al.A comparative study of growth performance and antioxidant status of rabbits when fed with or without chestnut tannins under high ambient temperature[J].Animal Feed Science and Technology,2011,164:89-95.
[12] 焦启扬,吴立军,黄建,等.板栗总苞化学成分的分离与鉴定[J].沈阳医科大学学报,2009,26(1):23-26.
[13] 张琳,高慧媛,马场正树,等.板栗总苞中的抗糖尿病活性成分[J].沈阳药科大学学报,2010(7):530-532,538.
[14] 李金凤,段玉清,马海乐,等.板栗壳中多酚的提取及体外抗氧化性研究[J].林产化学与工业,2010(2):53-58.
[15] 鲁晓翔,赵晨光,连喜军.板栗壳多酚提取条件及其抗氧化性研究[J].食品研究与开发,2008(3):32-36.
[16] 黄宏妙,李灵,郭占京,等.板栗毛壳水提液对大鼠脑缺血再灌注损伤后羟自由基、NO和GSH-Px的影响[J].时珍国医国药,2010(11):2917-2918.
[17] DIAO Q,QI G.Tannins in livestock feed in China //Tannins in Livestock and Human Nutrition.Proceedings of an international workshop on tannins in livestock and human nutrition.Adelaide: ,2003:76-80.
[18] LUO W,ZHAO M M,YANG B,et al.Identification of bioactive compounds in Phyllanthus emblica L.fruit and their free radical scavenging activities[J].Food Chemistry,2009,114:499-504.
[19] RE R,PELLEGRIEGRINI N,PROTEGGENTE A,et al.Antioxidant activity applying an improved ABTS radical cation decolorization assay[J].Free Radical Biology & Medicine,1999,26:1231-1237.
[20] SILVA A C D,JORGE N.Oxidative stability of soybean oil added to Lentinus edodes and Agaricus blazei mushrooms extracts in an accelerated storage test[J].Nutrition & Food Science,2012,42(1):34-40.
[21] 杜运平,徐浩,张宗和,等.板栗苞多酚提取条件研究[J].安徽农业科学,2011,39(2):828-829,839.
[22] DINIS L T,OLIVEIRA M M,ALMEIDA J,et al.Antioxidant activities of chestnut nut of Castanea sativa mill.(cultivar 'Judia') as function of origin ecosystem[J].Food Chemistry,2012,132:1-8.
