This study was conducted to analyze the expression patterns of calcium ion trans-membranous absorption related genes in gastrointestinal tract of Kunming mice. A total of 12 female Kunming mice aged 8 weeks with an average body weight of (30.71?2.93) g were used as experimental animals. Tissues of stomach, duodenum, jejunum, ileum, cecum and colon were collected for the analysis of vitamin D-dependent 9 ku calcium-binding protein (CaBP-D9k), transient receptor potential vanilloid receptor 6 (TRPV6), and vitamin D receptor (VDR) mRNA expression levels by RT-qPCR. The results showed as follows: 1) CaBP-D9k, TRPV6 and VDR mRNA expressed in stomach at a low level, but in cecum at a high level; 2) with the extension of small intestine, expression levels of CaBP-D9k and VDR mRNA were gradually reduced, while TRPV6 mRNA expressed in ileum at a high level; 3) with the extension of large intestine, expression levels of CaBP-D9k (P<0.05), VDR (P<0.05) and TRPV6 mRNA (P>0.05) were also gradually reduced at different levels. These results indicate that expression levels of CaBP-D9k, VDR and TRPV6 mRNA are associated with the capability of Ca2+ trans-membranous absorption in gastrointestinal tract.
YANG Yi
,
XIN Hailiang
,
XIA Xianlin
,
WU Wenxuan
,
LI Shengli
. Expression Patterns Analysis of Calcium Ion Trans-Membranous Absorption Related Genes in Gastrointestinal Tract of Kunming Mice[J]. Chinese Journal of Animal Nutrition, 2013
, 25(2)
: 441
-446
.
DOI: 10.3969/j.issn.1006-267x.2013.02.027
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