A Study of Insulin Action Mechanism on Casein Synthesis of Bovine Mammary Epithelial Cells

TIAN Qing; JI Yun; PANG Xueyan; WANG Hongrong

doi:10.3969/j.issn.1006-267x.2013.03.013

Chinese Journal of Animal Nutrition >

2013 , Vol. 25 >Issue 3: 550 - 560

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.03.013

Molecular Nutrition

A Study of Insulin Action Mechanism on Casein Synthesis of Bovine Mammary Epithelial Cells

TIAN Qing ,
JI Yun ,
PANG Xueyan ,
WANG Hongrong

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College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2012-09-07

Online published: 2013-02-28

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Abstract

This study was conducted to investigate the effects of different concentrations of insulin (INS) in medium on expression levels of α_s1-casein (CSN1S1) gene, mammalian target of rapamycin (mTOR) and Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway related genes. Holstein bovine mammary epithelial cells were treated with different concentrations (0, 2.5, 25.0, 250.0 and 5 000.0 ng/mL) of INS, respectively. The expression levels of CSN1S1 gene, mTOR and JAK-STAT signaling pathway related genes were determined by real-time quantitative PCR. The results showed as follows: compared with 0 ng/mL group, INS could promote the expression levels of CSN1S1, short-prolactin receptors (S-PRLR), protein kinase B (PKB) and eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) genes, and increase the expression levels of Janus kinase 2 (JAK2) and signal transducer and activator of transcription 5A (STAT5A) genes. Especially, the expression levels of these genes in 25 ng/mL group were the highest. The results indicate that INS can improve the expression level of CSN1S1 gene. The mechanism is as follows: firstly, INS can promote hormone receptor expression, and then promote genetic transcription; secondly, INS can promote the positive regulation expression of mTOR and JAK-STAT signaling pathways related genes, and highly improve the expression of CSN1S1 gene, which provides a material foundation for increasing milk protein synthesis at the translation level.

Key words： dairy cow; mammary epithelial cell; insulin; real-time quantitative PCR; mRNA

Cite this article

TIAN Qing , JI Yun , PANG Xueyan , WANG Hongrong . A Study of Insulin Action Mechanism on Casein Synthesis of Bovine Mammary Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2013 , 25(3) : 550 -560 . DOI: 10.3969/j.issn.1006-267x.2013.03.013

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