The objective of this experiment was to investigate the effects of dilution rate on nitrogen distribution in culture solution under the condition of continuous culture in vitro. A single factorial experiment was designed as 3 dilution rate levels (4%/h, 6%/h, and 8%/h, respectively) with 3 replicates for each dilution rate, and 5 goats fisted with cannulas were introduced as rumen liquor donors. The results showed as follows: the contents of protozoal protein, bacterial protein, soluble protein, peptide, free amino acid nitrogen and ammonia nitrogen in culture solution showed fluctuated changes with time going on, and the change trends were similar after re-feeding. With the dilution rate increasing, the contents of protozoal protein, bacterial protein, peptide, free amino acid nitrogen and ammonia nitrogen were all decreased. The significant or extremely significant differences were detected in all above indexes (P<0.05 or P<0.01) except for the soluble protein. However, the nitrogen quantities of above 6 indexes calculated with 24 hours were all increased with the dilution rate increasing, but the percentages of individual nitrogen quantity in the total nitrogen quantity were different from each other. The nitrogen quantity of protozoal protein and the percentage of that in the total nitrogen quantity under the dilution rate of 4%/h, 6%/h and 8%/h were 256.32 mg/d and 30%, 367.20 mg/d and 31%, 385.92 mg/d and 29%, respectively, and the 6%/h group had the highest percentage. The nitrogen quantity of bacterial protein and the percentage of that in the total nitrogen quantity under the dilution rate of 4%/h, 6%/h and 8%/h were 218.88 mg/d and 26%, 289.44 mg/d and 25%, 328.32 mg/d and 24%, respectively, and were decreased with the increase of dilution rate in their percentages. The nitrogen quantity of microbial protein (defined as the sum of protozoal protein and bacterial protein) and the percentage of that in the total nitrogen quantity under the dilution rate of 4%/h, 6%/h and 8%/h were 475.20 mg/d and 56%, 656.64 mg/d and 56%, 714.24 mg/d and 53%, respectively, and the quantity in the 6%/h group was higher and the percentage did not decrease in this group. The nitrogen quantity of soluble protein and the percentage of that in the total nitrogen quantity were increased with the dilution rate increasing, and those under the dilution rate of 4%/h, 6%/h and 8%/h were 178.56 mg/d and 21%, 263.52 mg/d and 22%, 345.60 mg/d and 26%, respectively. Under the different dilution rates, the percentage ranges of nitrogen quantities in free amino acid nitrogen, peptide and ammonia nitrogen in the total nitrogen quantity were 6%, 5% to 6% and 10% to 11%, respectively, but the percentages totally had a little change. It is concluded that dilution rate affects the quantity and the percentage distribution of nitrogen under the condition of continuous culture, with 6%/h dilution rate showing comparatively high quantity and percentage in microbial nitrogen.
WANG Mengzhi
,
WANG Shu
,
WANG Long
,
WANG Hongrong
. Effects of Dilution Rate on Nitrogen Distribution in Culture Solution under the Condition of Continuous Culture in Vitro[J]. Chinese Journal of Animal Nutrition, 2013
, 25(3)
: 561
-570
.
DOI: 10.3969/j.issn.1006-267x.2013.03.014
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