This experiment was conducted to investigate the effects of quercetin on endocrinic factor levels in adipocytes of Arbor Acres (AA) broilers. The methods of mechanical disruption and enzymic digestion were performed to isolate adipocytes from abdominal adipose tissue of 10-day-old AA broilers. The adipocytes were identified by oil red O staining and triglyceride (TG) content. The adipocytes were randomly allocated to 5 groups with 6 replicates each. Adipocytes were cultured in a basal medium (control), and the basal medium with 2‰ DMSO or 10, 20 and 40 mg/L quercetin. The cells and medium were collected after 24, 48, and 72 h of incubation. Enzymatic method was performed to determine TG content in adipocytes; radioactive immune method was performed to determine tumor necrosis factor (TNFα) content; ELISA was performed to determine the protein content of adiponectin (ADP) and peroxisome proliferator-activated receptor γ (PPARγ); and real-time polymerase chain reaction (RT-PCR) was performed to determine PPARγ gene mRNA expression. The results showed as follows: 1) there were abundant suspended cells in microscope through isolation of adipose tissue from AA broilers. The cells were identified as intracellular liquid drop stained by bright red and there was TG in cells. 2) Compared with control, TG content in adipocytes of AA broilers was significantly decreased when the basal medium supplemented with 10, 20, 40 μg/mL quercetin (P<0.05); the contents of TNFα and ADP in adipocytes were significantly increased by the supplementation of 10, 20 and 40 μg/mL quercetin in dose-dependent and time-dependent manner; mRNA expression and protein content of PPARγ were significantly inhibited by the supplementation of 10, 20 and 40 μg/mL quercetin in time-dependent manner but not in dose-dependent manner. The results indicate that the supplementation of quercetin can reduce TG content in adipocytes via regulating the contents of TNFα, ADP and PPARγ in adipocytes of AA broilers.