Short Communications

Fusion Expression of Chicken β-Defensin-9 and Preparation, Characterization of Its Polyclonal Antibody

  • XU Lanjiao ,
  • HONG Zhimin ,
  • SI Wei ,
  • LIU Baosheng ,
  • ZHANG Jinhua ,
  • LIU Siguo ,
  • LI Guanhong
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  • 1. College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China;
    2. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China

Received date: 2012-09-01

  Online published: 2013-02-28

Abstract

To prepare polyclonal antibody against chicken β-defensin-9 (AvBD9), AvBD9 gene was cloned into the prokaryotic expression vector pGEX-6P-1. The recombinant expression plasmid pGEX-6P-AvBD9 was constructed and then transformed into Escherichia coli (E. coli) to express AvBD9. The glutathione transferase-AvBD9 (GST-AvBD9) fusion protein was expressed in E. coli as an inclusion body. The fusion protein purified from the PAGE gel was used to immunize New Zealand white rabbit. The results showed that as detected by indirect ELISA and Western blot, polyclonal antibody against AvBD9 with the titer of 1:38 400 was obtained from the immunized rabbits.

Cite this article

XU Lanjiao , HONG Zhimin , SI Wei , LIU Baosheng , ZHANG Jinhua , LIU Siguo , LI Guanhong . Fusion Expression of Chicken β-Defensin-9 and Preparation, Characterization of Its Polyclonal Antibody[J]. Chinese Journal of Animal Nutrition, 2013 , 25(3) : 603 -608 . DOI: 10.3969/j.issn.1006-267x.2013.03.019

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