[1] VAN DIJK A,VELDHUIZEN E J,KALKHOVE S I,et al.The beta-defensin gallinacin-6 is expressed in the chicken digestive tract and has antimicrobial activity against food-borne pathogens[J].Antimicrobial Agents and Chemotherapy,2007,51(3):912-922.

[2] HARA S,YAMAKAWA M.Production in
Esherichia coli of moricin,a novel type antibacterial peptide from the silk worm,
Bombyx mori[J].Biochemical and Biophysical Research Communications,1996,220(3):664-669.

[3] KIM J,PARK J M,LEE B J.High-level expression and efficient purification of the antimicrobial peptide gaegurin 4 in
E.coli[J].Protein and Peptide Letters,1997,4(6):391-396.
[4] PIERCE J C,MALOY W L,SALVADOR L,et al.Recombinant expression of the antimicrobial peptide polyphemusin and its activity against the protozoan oyster pathogen
Perkinsus marinus[J].Molecular Marine Biology and Biotechnology,1997,6(3):248-259.
[5] LYNN D J,HIGGS R,GAINES S,et al.Bioinformatic discovery and initial characterization of nine novel antimicrobial peptides genes in the chicken[J].Immunogenetics,2004,56(3):170-177.

[6] XIAO Y,HUGHES A L,ANDO J,et al.A genome-wide screen identifies a single β-defensin gene cluster in the chicken:implications for the origin and evolution of mammalian defensins[J].BMC Genomics,2004,5(3):56-66.
[7] VAN DIJK A,VELDHUIZEN E A,HAAGSMAN H P.Avian defensins[J].Veterinary Immunology and Immunopathology,2008,124(1/2):1-18.
[8] DERACHE C,ESNAULT E,BONSERGENT C,et al.Differential modulation of β-defensin gene expression by
Salmonella Enteritidis in intestinal epithelial cells from resistant and susceptible chicken inbred lines[J].Developmental and Comparative Immunology,2009,33(9):959-966.

[9] EBERS K L,ZHANG C Y,ZHANG M Z,et al.Transcriptional profiling avian beta-defensins in chicken oviduct epithelial cells before and after infection with
Salmonella enterica serovar enteritidis[J].BMC Microbiology,2009(9):153-162.
[10] LAEMMLI U K.Cleavage of structural proteins during the assembly of the head of bacteriophage T4[J].Nature,1970,247:680-685.
[11] 萨姆布鲁克 J,拉塞尔D W.分子克隆实验指南[M].黄培堂,译.3版.北京:科学出版社,2002:1256-1259.
[12] 石建州.家禽β-防御素基因的克隆、原核表达及活性鉴定[D].硕士学位论文.郑州:河南农业大学,2007:58-63.