This experiment was conducted to determine the dietary zinc (Zn) requirement of spotted babylon, Babylonia areolate. Six doses of 0, 5, 10, 20, 30 and 50 mg/kg Zn from zinc sulfate were supplemented in a basal diet (casein and gelatin were used as protein sources) to prepare 6 experimental diets. In the 6 experimental diets, the Zn levels were 7.1, 10.1, 15.3, 23.4, 33.0 and 54.4 mg/kg, respectively. Each diet was fed one group (each group had 3 replicates and each replicate had 80 spotted babylon) of spotted babylon with an initial body weight of (106.25±0.00) mg for 8 weeks. The Zn content in rearing seawater was 5.74 μg/L. The results showed as follows: the survival rate (SR), feed conversion rate (FCR), protein efficiency rate (PER) and soft body to shell ratio (SB/SR) were not significantly affected by dietary Zn content (P>0.05). With the increase of dietary Zn content, the weight gain rate (WGR) was significantly increased firstly (P<0.05), and then there was no significant difference among all groups when dietary Zn content reached 15.3 mg/kg (P>0.05). 2) No significant differences were found in the contents of moisture, crude protein, ether extract and ash in soft body of spotted babylon among all groups (P>0.05). 3) The soft body Zn content of spotted babylon had a positive correlation with dietary Zn content (P<0.05, R2=0.975 0), but the shell Zn content was not significantly affected by dietary Zn content (P>0.05). 4) With the increase of dietary Zn content, the activities of total superoxide dismutase (T-SOD), copper-zinc superoxide dismutase (CuZn-SOD), catalase (CAT) and alkaline phosphatase (AKP) in visceral mass of spotted babylon were significantly increased firstly (P<0.05), and then no significant differences were found in above enzymic activities among all groups when dietary Zn content reached 23.4 mg/kg (P>0.05). Using the broken-line model with WGR as the dependent variable, the optimum dietary Zn requirement of spotted babylon is estimated to be 15.3 mg/kg. Analysis by broken-line of the activities of T-SOD, CuZn-SOD, CAT and AKP in visceral mass indicates that the optimum requirement of juvenile spotted babylon for dietary Zn is 22.9 to 24.3 mg/kg.
YANG Yuanzhi
,
WU Yeyang
,
DONG Xiaohui
,
TAN Beiping
,
YANG Qihui
,
CHI Shuyan
,
LIU Hongyu
. Dietary Zinc Requirement of Spotted Babylon, Babylonia areolate[J]. Chinese Journal of Animal Nutrition, 2013
, 25(3)
: 643
-650
.
DOI: 10.3969/j.issn.1006-267x.2013.03.024
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