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Effects of Water Temperature and Dietary Protein Level on Intestinal Digestive Enzyme Activities of Juvenile Songpu Mirror Carp (Cyprinus specularis Songpu)

  • HUANG Jinfeng ,
  • ZHAO Zhigang ,
  • LUO Liang ,
  • XU Qiyou
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  • 1. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;
    2. Fisheries and Life Science College, Shanghai Ocean University, Shanghai 201306, China

Received date: 2012-09-26

  Online published: 2013-02-28

Abstract

This experiment was conducted to investigate the effects of water temperature and dietary protein level on intestinal digestive enzyme activities of juvenile Songpu mirror carp (Cyprinus specularis Songpu). A total of 1 350 juvenile Songpu mirror carp with an average body weight of (10.11±1.07) g were randomly assigned to 15 groups with 3 replicates per group and 30 fish per replicate. Juvenile Songpu mirror carp were fed five diets containing 30.0%, 32.0%, 34.0%, 36.0% and 38.0% protein, respectively, and reared at different water temperatures (18, 23 and 28 ℃) for 60 days. The results showed as follows: water temperature significantly influenced the activities of protease, amylase and lipase in foregut, midgut and hindgut (P<0.05 or P<0.01). The protease activity in foregut, midgut and hindgut and foregut amylase activity were significantly influenced by dietary protein level (P<0.05 or P<0.01). In addition to foregut protease activity, the interaction effect between water temperature and dietary protein level was not significantly affected the activities of protease, amylase and lipase in each intestinal section (P>0.05). At the same water temperature, the activities of protease, amylase and lipase in intestine were firstly increased and then decreased with the dietary protein level increasing; at the same dietary protein level, the activities of protease and amylase in intestine were increased with the water temperature increasing, while the lipase activity in intestine was decreased. In conclusion, the water temperature and dietary protein level are closely related to the intestinal digestive enzyme activities of juvenile Songpu mirror carp. Based on the intestinal protease activity, the optimal dietary protein requirements for juvenile Songpu mirror carp in different water temperatures are 35.83% to 36.96% at 18 ℃, 37.40% to 39.58% at 23 ℃, and 36.79% to 39.99% at 28 ℃.

Cite this article

HUANG Jinfeng , ZHAO Zhigang , LUO Liang , XU Qiyou . Effects of Water Temperature and Dietary Protein Level on Intestinal Digestive Enzyme Activities of Juvenile Songpu Mirror Carp (Cyprinus specularis Songpu)[J]. Chinese Journal of Animal Nutrition, 2013 , 25(3) : 651 -660 . DOI: 10.3969/j.issn.1006-267x.2013.03.025

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