Effects of Vitamin D3 Supplemental Level on Cell Proliferation and Apoptosis of Peripheral Immune Related Tissues in Monopterus albus

LI Debing; LI Chao; ZHANG Gongwei; PENG Xi; ZHOU Dinggang; LI Xuewei

doi:10.3969/j.issn.1006-267x.2013.04.012

2013 , Vol. 25 >Issue 4: 752 - 760

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.04.012

Aquaculture Nutrition

Effects of Vitamin D₃ Supplemental Level on Cell Proliferation and Apoptosis of Peripheral Immune Related Tissues in Monopterus albus

LI Debing ,
LI Chao ,
ZHANG Gongwei ,
PENG Xi ,
ZHOU Dinggang ,
LI Xuewei

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1. College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, China;
2. College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China

Received date: 2012-11-02

Online published: 2013-03-25

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Abstract

This experiment was conducted to study the effects of vitamin D₃ supplemental level on cell proliferation and apoptosis of peripheral immune related tissues in Monopterus albus. A total of 360 healthy M. albus with an average body weight of (21.7?2.1) g were randomly assigned to 6 groups with 2 replicates per group and 30 M. albus per replicate. The M. albus in the 6 groups were fed the basal diet supplemented with 0 (control), 250, 500, 1 000, 2 000 and 4 000 IU/kg vitamin D₃, respectively. The experiment lasted for 60 days, and the hepatopancreas, head kidney, spleen and hindgut of 6 M. albus in each group were collected after feeding experiment. The proliferation and apoptosis of cells in each sample were tested by flow cytometry. The results showed as follows: the change trends of the cell proliferation and apoptosis of spleen, hindgut and head kidney tissues were basically identical with the vitamin D₃ supplemental level increasing, which presented as the 1 000 IU/kg group had the highest cell proliferating index (PI) and the lowest cell apoptotic index (AI), and there were significant differences between 1 000 IU/kg group and control group, 4 000 IU/kg group (P<0.05). The PI of hepatopancreas cells was decreased with the increase of vitamin D₃ supplemental level, and the lowest PI was found in 4 000 IU/kg group, in which the cell PI was significantly lower than that in the control group (P<0.05). The lowest AI of hepatopancreas cells was found in 1 000 IU/kg group, in which the cell AI was significantly lower than that in control group and 4 000 IU/kg group (P<0.05). It can be concluded that 1 000 IU/kg vitamin D₃ supplementation reduces the cell apoptosis of peripheral immune related tissues, promotes the cell proliferation of head kidney, spleen and hindgut, and it is beneficial for the development of peripheral lymphoid organs in M. albus; 4 000 IU/kg of vitamin D₃ supplementation hinders the cell proliferation and accelerates the cell apoptosis of peripheral immune related tissues, and induces the inhibition of immune function in M. albus.

Key words： Monopterus albus; vitamin D₃; cell proliferation; cell apoptosis

Cite this article

LI Debing , LI Chao , ZHANG Gongwei , PENG Xi , ZHOU Dinggang , LI Xuewei . Effects of Vitamin D₃ Supplemental Level on Cell Proliferation and Apoptosis of Peripheral Immune Related Tissues in Monopterus albus[J]. Chinese Journal of Animal Nutrition, 2013 , 25(4) : 752 -760 . DOI: 10.3969/j.issn.1006-267x.2013.04.012

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