Aflatoxin B1 Affects Growth, Liver Tissue Structure and Immune Related Gene Expression of Meat Ducklings

LV Wuxing; HE Jianhua; SONG Hongguo; LIU Minmin; JIN Xueli; LI Junbo

doi:10.3969/j.issn.1006-267x.2013.04.019

Chinese Journal of Animal Nutrition >

2013 , Vol. 25 >Issue 4: 812 - 818

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.04.019

Feed Security

Aflatoxin B₁ Affects Growth, Liver Tissue Structure and Immune Related Gene Expression of Meat Ducklings

LV Wuxing ,
HE Jianhua ,
SONG Hongguo ,
LIU Minmin ,
JIN Xueli ,
LI Junbo

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1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
2. Research and Development Center of Tangrenshen Group, Zhuzhou 412007, China

Received date: 2012-10-08

Online published: 2013-03-25

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Abstract

The objective of present study was to research the effects of aflatoxin B₁ (AFB₁) on the growth, liver tissue structure, and the expression of interleukin-2 (IL-2) and interferon-γ (IFN-γ) genes of meat ducklings. A total of 240 healthy Shuangguitou meat ducklings aged 1 day were randomly allotted to 4 groups with 6 replicates in each group and 10 ducklings in each replicate. The experiment was conducted for four weeks. The ducklings in control group were fed a basal diet, and those in test groups Ⅰ, Ⅱ and Ⅲ were fed the basal diet with 50, 100 and 150 μg/kg AFB₁, respectively. The ducklings were weighed and the consumption of feed was calculated. At day 28, ducklings from each group were randomly sampled and slaughtered for determining pathological section and the expression of IL-2 and IFN-γ genes. The results showed as follows: 1) the supplementation of AFB₁ significantly decreased average daily gain (ADG) and average daily feed intake (ADFI) of ducklings (P<0.01). Compared with the control group, the final body weight of test groups Ⅰ, Ⅱ and Ⅲ was significantly decreased by 14.67% (P<0.01), 30.38% (P<0.01) and 31.83% (P<0.01). The supplementation of 100 and 150 μg/kg AFB₁ significantly increased feed/gain (F/G) (P<0.01), and significantly increased mortality (P<0.05). 2) With the increase of AFB₁ supplemental level, the pathological changes in liver were more and more serious, and the mRNA expression of IL-2 and IFN-γ genes in liver was reduced to some degree, and the supplementation of 100 and 150 μg/kg AFB₁ significantly decreased the mRNA expression of IFN-γ gene in liver (P<0.05). In conclusion, the supplementation of AFB₁ can decrease performance and induce pathological changes in liver, and decrease the expression of IL-2 and IFN-γ genes in liver, and then cause the immunosuppression of ducklings.

Key words： AFB₁; performance; liver tissue; IL-2 gene; IFN-γ gene; ducklings

Cite this article

LV Wuxing , HE Jianhua , SONG Hongguo , LIU Minmin , JIN Xueli , LI Junbo . Aflatoxin B₁ Affects Growth, Liver Tissue Structure and Immune Related Gene Expression of Meat Ducklings[J]. Chinese Journal of Animal Nutrition, 2013 , 25(4) : 812 -818 . DOI: 10.3969/j.issn.1006-267x.2013.04.019

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