This experiment was conducted to investigate the effects of fermented soybean meal on growth performance, serum biochemical indices, mucosal immune function and microorganism in yellow-feathered broilers. A total of 250 one-day-old yellow-feathered broilers were randomly divided into five groups, and each group included five replicates (10 broilers per replicate). Control group (group Ⅰ) was fed a basal diet.Group Ⅱ was fed the basal diet supplemented with 15% zinc bacitracin 200 mg/kg and 10% colistin sulfate 60 mg/kg. The treatment groups (groups Ⅲ, Ⅳ, Ⅴ) were fed the basal diet replaced some of common soybean meal with 3%, 6% and 9% fermented soybean meal,respectively. The experiment lasted for 28 days. The results showed as follows: 1) compared with group Ⅰ, the body weight gain of groups Ⅳ and Ⅴ was significantly increased by 3.87% and 4.38% (P<0.05), respectively; the feed/gain of groups Ⅳ and Ⅴ was significantly reduced by 4.75% (P>0.05) and 5.71% (P<0.05), respectively; the rate of survival of group Ⅴ was significantly increased by 6.38% (P<0.01). 2) The activity of alkaline phosphatase in serum of groups Ⅱ and Ⅴ was significantly higher than that of group Ⅰ (P<0.05), uric acid content in serum of group Ⅴ was significantly lower than that of group Ⅰ (P<0.05), and the other groups were no significant differences (P>0.05). 3) Compared with group Ⅰ, duodenum villus height of groups Ⅱ, Ⅲ, Ⅳ and Ⅴ was significantly higher than that of group Ⅰ (P<0.01), and group Ⅴ was the highest. The number of mast cells of group Ⅴ was significantly decreased compared with group Ⅰ (P<0.01). The number of secretory immunoglobulin A (sIgA) positive cells of groups Ⅱ, Ⅲ, Ⅳ and Ⅴ was significantly increased compared with group Ⅰ (P<0.01), and group Ⅴ was the highest in all. 4) The number of Escherichia coli of groups Ⅱ and Ⅴ was significantly lower than that of groups Ⅰ, Ⅲ and Ⅳ (P<0.05), and the number of Lactobacillus of group Ⅴ was significantly increased compared with group Ⅰ (P<0.05). It is concluded that fermented soybean meal can improve the body weight gain and the activity of alkaline phosphatase in serum, decrease feed/gain and uric acid content in serum, increase duodenal villus height, make the number of mast cells and sIgA-positive cells stable and intestinal micro environment better, reduce the number of Escherichia coli in cecum, and increase the number of Lactobacillus in yellow-feathered broilers and the best replacement rate in prior period is 9%.
XU Lihui
,
QI Ruixue
,
WANG Changkang
,
WANG Quanxi
,
XIE Liqu
,
LIN Lihua
,
CHEN Qingda
. Effects of Fermented Soybean Meal on Growth Performance, Serum Biochemical Indices, Mucosal Immune Function and Microorganism in Yellow-Feathered Broilers[J]. Chinese Journal of Animal Nutrition, 2013
, 25(4)
: 840
-848
.
DOI: 10.3969/j.issn.1006-267x.2013.04.023
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