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Optimization of Removal Method of Glycinin Immunogenicity by Glycosylation

  • TAN Zhen ,
  • SUN Zewei
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  • College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China

Received date: 2012-10-26

  Online published: 2013-03-25

Abstract

To optimize the removal method of glycinin immunogenicity by glycosylation, a dry heat way glycosylation reaction by fructo-oligosaccharide and rough glycinin was adopted in this study to select the optimal group. Different temperature and humidity conditions were selected, and the dissolved protein and the removal efficiency of immunogenicity were determined during 0 to 120 hours. The results showed that protein-dissolved level decreased, but immunogenicity removal increased in all products as time went on, and under the condition of 65 ℃, 75% relative humidity and molar ratio of protein to sugar at 1: 14, after 60-hour response time, protein-dissolved level and immunogenicity removal were the ideal results. In conclusion, this condition can be used as the optimized dry heat method about glycosylation reaction to remove glycinin immunogenicity.

Cite this article

TAN Zhen , SUN Zewei . Optimization of Removal Method of Glycinin Immunogenicity by Glycosylation[J]. Chinese Journal of Animal Nutrition, 2013 , 25(4) : 884 -890 . DOI: 10.3969/j.issn.1006-267x.2013.04.029

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