Dietary Folic Acid Level Affects Growth Performance, Serum Biochemical Parameters, Enzyme Activity and MTHFR Gene Expression of Geese

MENG Lingfeng; WANG Baowei; GE Wenhua; ZHANG Ming; YUE Bin; WANG Jiao; WANG Di; CHEN Miaolu

doi:10.3969/j.issn.1006-267x.2013.05.013

2013 , Vol. 25 >Issue 5: 985 - 995

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.05.013

Poultry Nutrition

Dietary Folic Acid Level Affects Growth Performance, Serum Biochemical Parameters, Enzyme Activity and MTHFR Gene Expression of Geese

MENG Lingfeng ,
WANG Baowei ,
GE Wenhua ,
ZHANG Ming ,
YUE Bin ,
WANG Jiao ,
WANG Di ,
CHEN Miaolu

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Institute of High Quality Waterfowl, Qingdao Agricultural University, Qingdao 266109, China

Received date: 2012-10-30

Online published: 2013-04-12

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Abstract

This experiment was conducted to study the effects of dietary folic acid level on growth performance, serum biochemical parameters and enzyme activity and methylenetetrahydrofolate reductase (MTHFR) gene expression of geese, and to determine the folic acid optimum supplemental level of geese aged 1 to 4 weeks (brooding period) and 5 to 15 weeks (finishing period). Three hundred and sixty 1-day-old Qingnonghui geese were randomly selected and divided into 6 groups with 6 replicates per group and 10 geese per replicate. Geese in the six groups were fed the basal diet supplemented with 0 (control), 1, 2, 4, 8 and 16 mg/kg folic acid, respectively. The experiment lasted for 15 weeks. The results showed as follows: 1) dietary folic acid significantly increased average daily weight gain of geese aged 1 to 4 weeks and 5 to 15 weeks (P<0.05, P<0.01), significantly decreased feed to gain of geese aged 1 to 4 weeks (P<0.05), and reduced mortality rate. 2) Dietary folic acid significantly decreased serum glucose content of geese aged 1 to 4 weeks (P<0.05), and significantly increased serum triglyceride content (P<0.05), and the supplementation of 8 mg/kg folic acid significantly decreased serum urea nitrogen content (P<0.05). The supplementation of 16 mg/kg folic acid significantly decreased serum urea nitrogen content of geese aged 5 to 15 weeks(P<0.05). The supplementation of 2 to 16 mg/kg folic acid significantly decreased serum homocysteic acid content of geese aged 1 to 4 weeks and 5 to 15 weeks (P<0.05). 3) The supplementation of 1 and 2 mg/kg folic acid significantly increased serum MTHFR activity of geese aged 1 to 4 weeks (P<0.05), and significantly decreased serum dihydrofolate reductase (DHFR) activity (P<0.01 or P<0.05), and the supplementation of 2 mg/kg folic acid significantly decreased serum glutamic-oxaloacetic transaminase and lactate dehydrogenase activity (P<0.05). The supplementation of 2 mg/kg folic acid significantly increased serum MTHFR activity of geese aged 5 to 15 weeks (P<0.05). 4) In the brooding period, the MTHFR gene expression was significantly positive correlation with MTHFR activity (P<0.05), and significantly negative correlation with DHFR activity (P<0.05). In the finishing period, the MTHFR gene expression was significantly negative correlation with MTHFR and glutamic pyruvic transaminase activity (P<0.05). It is conclusion that: 1) according to the regression equation between growth performance and dietary folic acid level, the suggestion of optimum dietary folic acid level is 2.45 mg/kg in brooding period, and 2.08 mg/kg in finishing period. Dietary folic acid can decrease mortality rate. 2) Dietary folic acid level has an important role in the regulation of serum biochemical parameters and enzyme activity. 3) Folic acid has a direct impact on MTHFR gene expression in geese liver, and MTHFR gene expression is closely related to the activities of MTHFR, DHFR and glutamic pyruvic transaminase.

Key words： folic acid; geese; growth performance; serum biochemical parameters; enzyme activity; MTHFR gene expression

Cite this article

MENG Lingfeng , WANG Baowei , GE Wenhua , ZHANG Ming , YUE Bin , WANG Jiao , WANG Di , CHEN Miaolu . Dietary Folic Acid Level Affects Growth Performance, Serum Biochemical Parameters, Enzyme Activity and MTHFR Gene Expression of Geese[J]. Chinese Journal of Animal Nutrition, 2013 , 25(5) : 985 -995 . DOI: 10.3969/j.issn.1006-267x.2013.05.013

References

[1] 李红毅,白树民,朱德兵,等.不同叶酸、维生素B₆摄入水平对模拟失重大鼠Hcy代谢的影响[J].营养学报,2010,32(5):480-483.

[2] ABAS I,KAHRAMAN R,ESECELI H,et al.The effect of high levels of folic acid on performance and egg quality of laying hens fed on diets with and without ascorbic acid from 28-36 weeks of age[J].Journal of Animal and Veterinary Advances,2008,7(4):389-395.

[3] El-HUSSEINY M A,ABO-EL-ELLA M O,ABD-ELSAMEEMAGD A M,et al.Response of broilers performance to dietary betaine and folic acid at different methionine levels[J].International Journal of Poultry Science,2010,6(7):515-523.

[4] WHITEHEAD C C,MCCORMRCK H A,RENNIE J S,et al.Folic acid requirements of broilers[J].Israel Journal of Veterinary Medicine,1997,52(1):12-16.

[5] 杨光波,陈代文,余冰.叶酸水平对断奶仔猪生长性能及血清组织中蛋白质代谢的指标影响[J].中国畜牧杂志,2011,47(5):24-28.

[6] 丑武江,葛文霞,木沙江·乌不力.烟酸和不同水平叶酸对肉仔鸡生产性能的影响[J].中国畜牧兽医,2009,36(6):23-26.

[7] DORA A,ROTH-MAIER,BARBARA M.Fortification of eggs with folic acid as possible contribution to enhance the folic acid status of populations[J].International Journal for Vitamin and Nutrition Research,2007,77(4):297-300.

[8] RYU K S,ROBERSON K D,PESTI G M,et al.The folic acid requirements of starting broiler chicks fed diets based on practical ingredients.2.Interrelationships with dietary methionine[J].Poultry Science,1995,74(9):1456-1462.

[9] EL-HUSSEINY A Z,SOLIMAN I I,OMARA H M R,et al.Evaluation of dietary methionine,folic acid and cyanocobalamin (B₁₂) and their interactions in laying hen performance[J].International Journal of Poultry Science,2008,7(5):461-469.

[10] 江锋,吴鸿跃.补充叶酸对2型糖尿病患者的血糖控制和胰岛素抵抗的影响[J].中国医学创新,2012,9(13):34-37.

[11] ESECELI H,DEGRIMENCIOGLU N,BILGIC M.The effect of inclusion of chromium yeast (Co-Fator Ⅱ,Alltech Inc.) and folic acid to the rations of laying hens on performance,egg quality,egg yolk cholesterol,folic acid and chromium levels[J].Journal of Animal and Veterinary advances,2010,9(2):384-391.

[12] WANG S P,YIN Y L,QIAN Y,et al.Effects of folic acid on the performance of suckling piglets and sows during lactation[J].Science of Food and Agriculture,2011,91:2371-2377.

[13] 姚英,陈代文,刘静波,等.叶酸对超早期断奶宫内发育迟缓仔猪肝脏结构和细胞凋亡相关基因表达的影响[J].动物营养学报,2012,24(2):271-279.

[14] 丑武江,郭雄全,葛文霞.叶酸对肉仔鸡蛋白质和生产性能影响的研究[J].新疆农业科学,2009,46(5):1140-1143.

[15] 任明保,柴晓芮.叶酸代谢异常与Down综合征的风险因素[J].国外医学遗传学分册2002,25(6):341-343.

[16] 吕风华,高建芝,崔佳佳,等.叶酸对冠心病大鼠血清Hcy和血管内皮生长因子的影响[J].西安交通大学学报:医学版,2011,32(4):449-452.

[17] TACTACAN G B,RODRIGUEZ-LECOPTE J C O K,HOUSE J D.The adaptive transport of folic acid in the intestine of laying hens with increased supplementation of dietary folic acid[J].Poultry Science,2012,91(1):121-128.

[18] HEBERT K,HOUSE J D,GUENTER W.Effect of dietary folic acid supplementation on egg folic content and the performance and folic status of two strains of laying hens[J].Poultry Science,2005,84(10):1533-1538.

[19] ANSARI M N,NIGAM G K,BHANDARI U.Effect of folic acid on hematological changes in methionine-induced hyperhomocysteinemia in rats[J].Pharm Science,2009,71:270-275.

[20] 钱瑛,王之盛,孔祥峰,等.叶酸对哺乳仔猪生长性能及母猪血清生化指标的影响[J].天然产物研究与开发,2008,20:497-504.

[21] 段赛星,陈兴乾,杨膺白,等.叶酸对圈养隆林黑山羊血清蛋白质的影响研究[J].现代农业科技,2009,9:228-229.

[22] 黄瀚,李兵,欧阳林旗.叶酸受体及二氢叶酸还原酶与人乳腺癌细胞MCF-7/ADR多药耐药的关系[J].中国药理学通报,2011,27(1):99-103.

[23] 刘玉兰,肖文斌,张国艳.A型萎缩性胃炎血清乳酸脱氢酶及α-羟丁酸的研究[J].中华消化杂志,2002,22(4):220-223.

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