Effects of &beta;-Glucan and Selenium, Vitamin E Combined Supplementation on Tissue Biochemical Indexes and Immune, Antioxidant Related Enzyme mRNA Expression of Litopenaeus vannamei

LIU Qunfang; CAO Junming; HUANG Yanhua; WANG Guoxia; WEN Yuanhong; ZHOU Tingting; SUN Zhiwu; LIU Xiaoling

doi:10.3969/j.issn.1006-267x.2013.05.020

2013 , Vol. 25 >Issue 5: 1045 - 1053

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.05.020

Molecular Nutrition

Effects of β-Glucan and Selenium, Vitamin E Combined Supplementation on Tissue Biochemical Indexes and Immune, Antioxidant Related Enzyme mRNA Expression of Litopenaeus vannamei

LIU Qunfang ,
CAO Junming ,
HUANG Yanhua ,
WANG Guoxia ,
WEN Yuanhong ,
ZHOU Tingting ,
SUN Zhiwu ,
LIU Xiaoling

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1. College of Animal Science, South China Agricultural University, Guangzhou 510642, China;
2. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
3. Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China;
4. Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China

Received date: 2012-11-15

Online published: 2013-04-12

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Abstract

This experiment was conducted to investigate the effects of β-glucan (βG) and selenium (Se), vitamin E on tissue biochemical indexes and immune, antioxidant related enzyme mRNA expression of Litopenaeus vannamei. Six experimental diets were formulated with different levels of βG, Se (selenium methionine form) and vitamin E (vitamin E acetate form), in which the doses and combinations of βG, Se and vitamin E were 0, 300 mg/kg βG, 300 mg/kg βG+0.2 mg/kg Se, 300 mg/kg βG+100 mg/kg vitamin E, 300 mg/kg βG+0.2 mg/kg Se+100 mg/kg VE, and named G0, G1, G2, G3 and G4, respectively. A total of 800 juvenile shrimp with an average body weight of (0.83±0.02) g were randomly assigned to 5 groups with 4 replicates per group and 40 shrimps per replicate. The shrimps in each group were randomly fed one diet, and the experiment lasted for 5 weeks. The results showed as follows: compared with G0 group, serum total protein content in G1 and G4 groups was significantly decreased (P<0.05), and serum uric acid content and alanine aminotransferase activity in G4 group were significantly increased (P<0.05). Serum total cholesterol, triglycerides, glucose contents and aspartate aminotransferase activity, and hepatopancreas total protein content, alanine aminotransferase and aspartate aminotransferase activities were not significantly different among 5 groups (P>0.05). The hepatopancreas prophenoloxidase mRNA relative expression in G4 group was significantly higher than that in other groups (P<0.05), and that in G2 group was significantly higher than that in G1 group (P<0.05). Compared with the G0 group, hepatopancreas lysozyme mRNA relative expression in G1, G2, G3 and G4 groups was not significantly different (P>0.05), while that in G2, G3 and G4 groups showed a decreased tendency compared with G1 group, and that in G4 group was significantly decreased (P<0.05). Superoxide dismutase mRNA relative expression in G2 group had the highest value, and significantly higher than that in G3 group (P<0.05). The highest value of glutathione peroxidase mRNA relative expression was found in G4 group, and significantly higher than that in G0, G1 and G3 groups (P<0.05), while there was no significant difference between G4 and G2 groups (P>0.05). It is concluded that βG and Se combined supplementation can enhance the immune defence function of Litopenaeus vannamei, and can be used as the compound immunization for Litopenaeus vannamei.

Key words： Litopenaeus vannamei; β-glucan; selenium; vitamin E; tissue biochemical indexes; mRNA expression

Cite this article

LIU Qunfang , CAO Junming , HUANG Yanhua , WANG Guoxia , WEN Yuanhong , ZHOU Tingting , SUN Zhiwu , LIU Xiaoling . Effects of β-Glucan and Selenium, Vitamin E Combined Supplementation on Tissue Biochemical Indexes and Immune, Antioxidant Related Enzyme mRNA Expression of Litopenaeus vannamei[J]. Chinese Journal of Animal Nutrition, 2013 , 25(5) : 1045 -1053 . DOI: 10.3969/j.issn.1006-267x.2013.05.020

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