Effects of Seleno-Chitosan on Tissue Selenium Content, Activities and Gene Expressions of Selenoenzymes in Breeder Cocks

ZHAO Xiaoxu; HUA Jing; WANG Xiaoxia; ZHANG Jie

doi:10.3969/j.issn.1006-267x.2013.05.025

2013 , Vol. 25 >Issue 5: 1085 - 1092

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.05.025

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Effects of Seleno-Chitosan on Tissue Selenium Content, Activities and Gene Expressions of Selenoenzymes in Breeder Cocks

ZHAO Xiaoxu ,
HUA Jing ,
WANG Xiaoxia ,
ZHANG Jie

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1. College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China;
2. College of Biological Science and Engineering, Beijing University of Agriculture, Beijing 102206, China;
3. District Commission of Beijing's Changping District, Beijing 102200, China;
4. Animal Husbandry and Veterinary Department, Beijing Vocational College of Agriculture, Beijing 102442, China

Received date: 2012-12-17

Online published: 2013-04-12

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Abstract

This experiment was conducted to compare the effects of seleno-chitosan (SC) and sodium selenite (SS) on tissue selenium retention, thioredoxin reductase (TrxR) and iodothyronine deiodinase Ⅰ (IDⅠ) activities and mRNA relative expression levels of TrxR2 and IDⅠ genes in Hy-Line breeder cocks. Two hundred and eighty 20-week-old Hy-Line breeder cocks were allocated randomly into seven treatments with four replicates per treatment and ten breeder cocks per replicate. Control group was fed a basal diet, while the six other groups were fed the basal diet supplemented with 0.4, 0.8 and 1.2 mg/kg selenium from SC or SS, respectively. The experiment lasted for 35 days. The results showed as follows: 1) Se source and supplemental level did not influence the growth performance of breeder cocks (P>0.05). 2) The selenium content in testis of breeder cocks of 1.2 mg/kg SS supplemental group and 1.2 mg/kg SC supplemental group was significantly higher than that of control group (P<0.05 and P<0.01, respectively). The selenium content in kidney of breeder cocks of 0.8 mg/kg SS supplemental group, 1.2 mg/kg SS supplemental group and 1.2 mg/kg SC supplemental group had a significant increase compared with control group (P<0.05). 3) TrxR activity in testis of breeder cocks of 0.4 mg/kg SS supplemental group and 0.4 mg/kg SC supplemental group was significantly higher than that of control group (P<0.01 and P<0.05, respectively), but only IDⅠ activity in kidney of 0.4 mg/kg SC supplemental group was significantly higher than that of control group (P<0.01). 4) The mRNA relative expression level of TrxR2 gene in testis of breeder cocks of 0.4 mg/kg SC supplemental group was the highest of all groups, but there was no significant difference among all groups (P>0.05). With the increase of supplemental selenium level, the mRNA relative expression level of TrxR2 gene in testis was increased at first, and then descended in SS supplemental groups, while the mRNA relative expression level of TrxR2 gene was increased at first, then descended and increased at last in SC supplemental groups. The mRNA relative expression level of IDⅠ gene in kidney of breeder cocks of 0.4 mg/kg SS supplemental group was significantly lower than that of control group (P<0.05). These results indicate that SC has greater effects on the deposition of tissue Se, TrxR and IDⅠ activities and mRNA relative expression levels of TrxR2 and IDⅠ genes in Hy-Line breeder cocks, and supplementing SC at a lower level (0.4 mg/kg selenium) is better.

Key words： seleno-chitosan; tissue selenium content; thioredoxin reductase; iodothyronine deiodinase Ⅰ; gene expression

Cite this article

ZHAO Xiaoxu , HUA Jing , WANG Xiaoxia , ZHANG Jie . Effects of Seleno-Chitosan on Tissue Selenium Content, Activities and Gene Expressions of Selenoenzymes in Breeder Cocks[J]. Chinese Journal of Animal Nutrition, 2013 , 25(5) : 1085 -1092 . DOI: 10.3969/j.issn.1006-267x.2013.05.025

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