Effects of Dietary Cello-Oligosaccharide on Growth Performance, Colonic Microflora and Intestinal Mucosal Permeability of Growing Pigs

XU Lourong; LUAN Zhaoshuang; HU Caihong; SHI Bo

doi:10.3969/j.issn.1006-267x.2013.06.021

2013 , Vol. 25 >Issue 6: 1293 - 1298

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.06.021

Feed Science and Technology

Effects of Dietary Cello-Oligosaccharide on Growth Performance, Colonic Microflora and Intestinal Mucosal Permeability of Growing Pigs

XU Lourong ,
LUAN Zhaoshuang ,
HU Caihong ,
SHI Bo

Expand

1. Key Laboratory of Molecular Animal Nutrition of Ministry of Education, Institute of Feed Science, Zhejiang University, Hangzhou 310058, China;
2. Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2012-12-24

Online published: 2013-05-15

Fold

Abstract

This experiment was conducted to investigate the effects of dietary cello-oligosaccharide on growth performance, colonic microflora and intestinal mucosal permeability of growing pigs. A total of 96 growing pigs (Duroc×Landrace×Yorkshire) with an average body weight of 20 kg were randomly allocated into 4 groups with 3 replicates per group and 8 pigs per replicate. Pigs in the control group were fed a basal diet, and pigs in the experimental groups were fed the basal diet supplemented with 0.1%, 0.2% and 0.3% cello-oligosaccharide, respectively. The experiment lasted for 42 days. The results showed as follows: compared with the control group, pigs fed diets with 0.2% cello-oligosaccharide had the best performance, the average daily gain was significantly increased by 7.51% (P<0.05), and feed/gain was significantly decreased by 5.93% (P<0.05); the supplementation of 0.2% cello-oligosaccharide significantly increased the number of Bifidobacterium and Lactobacillus (P<0.05), and significantly reduced the number of Escherichia coli and Clostridium in colon (P<0.05); through the Ussing chamber technology, pigs fed diets with 0.2% cello-oligosaccharide had higher transepithelial electrical resistance (TER) and lower mucosal-to-serosal flux of 4 ku-fluorescein isothiocyanate-dextran (FD4) permeability in colonic epithelium (P<0.05). The supplementation of 0.3% cello-oligosaccharide had significant effects on above indexes (P<0.05), but there was no significant difference between 0.3% and 0.2% cello-oligosaccharide supplementation (P>0.05). The supplementation of 0.1% cello-oligosaccharide did not affect above indexes (P>0.05). In conclusion, the supplementation of cello-oligosaccharide can significantly improve the growth performance, colonic microflora and colonic mucosal barrier function of growing pigs. The optimal supplemental level of cello-oligosaccharide for growing pigs is 0.2%.

Key words： cello-oligosaccharide; growth performance; intestinal mucosal barrier; growing pigs

Cite this article

XU Lourong , LUAN Zhaoshuang , HU Caihong , SHI Bo . Effects of Dietary Cello-Oligosaccharide on Growth Performance, Colonic Microflora and Intestinal Mucosal Permeability of Growing Pigs[J]. Chinese Journal of Animal Nutrition, 2013 , 25(6) : 1293 -1298 . DOI: 10.3969/j.issn.1006-267x.2013.06.021

References

[1] 罗佳捷,张彬,王洁.功能性寡糖在动物生产中的应用研究进展[J].广东畜牧兽医科技,2011(2):6-8.

[2] 刘程程.β-葡聚糖酶水解紫花苜蓿制备纤维寡糖的研究[D].硕士学位论文.北京:中国农业科学院,2011:2-8.

[3] YANG C M,FERKET P R,HONG Q H,et al.Effect of chito-oligosaccharide on growth performance,intestinal barrier function,intestinal morphology and cecal microflora in weaned pigs[J].Journal of Animal Science,2012,90(10):2671-2676.

[4] ZHAO P Y,JUNG J H,KIM I H.Effect of mannan oligosaccharides and fructan on growth performance,nutrient digestibility,blood profile,and diarrhea score in weanling pigs[J].Journal of Animal Science,2012,90(3):833-839.

[5] 游金明,付建福,王自蕊,等.丁酸钠和甘露寡糖对断奶仔猪生长性能和免疫功能的影响[J].动物营养学报,2010,22(2):346-351.

[6] OTSUKA M,ISHIDA A,NAKAYAMA Y,et al.Dietary supplementation with cello-oligosaccharide improves growth performance in weanling pigs[J].Animal Science Journal,2004,75(3):225-229.

[7] XIA M S,HU C H,XU Z R.Effects of copper bearing montmorillonite on the growth performance,intestinal microflora and morphology of weanling pigs[J].Animal Feed Science and Technology,2005,118(3/4):307-317.

[8] MOESER A J,RYAN K A,NIGHOT P K,et al.Gastrointestinal dysfunction induced by early weaning is attenuated by delayed weaning and mast cell blockade in pigs[J].American Journal of Physiology:Gastrointestinal and Liver Physiology,2007,293(2):G413-G421.

[9] OVERMAN E L,RIVIER J E,MOESER A J.CRF induces intestinal epithelial barrier injury via the release of mast cell proteases and TNF-alpha[J].PLoS One,2012,7(6):e39935.

[10] 莫正海.甘露寡糖的应用及其在断奶仔猪和肉鸡生产上的适宜添加量[J].现代农业科技,2010(7):346-347.

[11] HERFEL T M,JACOBI S K,LIN X,et al.Polydextrose enrichment of infant formula demonstrates prebiotic characteristics by altering intestinal microbiota,organic acid concentrations,and cytokine expression in suckling piglets[J].The Journal of Nutrition,2011,141(12):2139-2145.

[12] BERGMANN H,ROGOLL D,SCHEPPACH W,et al.The Ussing type chamber model to study the intestinal transport and modulation of specific tight-junction genes using a colonic cell line[J].Molecular Nutrition & Food Research,2009,53(10):1211-1225.

[13] 孙志洪,贺志雄,张庆丽,等.尤斯灌流系统在动物胃肠道屏障及营养物质转运中的应用[J].动物营养学报,2010,22(3):511-518.

[14] HU C H,GU L Y,LUAN Z S,et al.Effects of montmorillonite-zinc oxide hybrid on performance,diarrhea,intestinal permeability and morphology of weanling pigs[J].Animal Feed Science and Technology,2012,177(1/2):108-115.

[15] HU C H,SONG J,YOU Z T,et al.Zinc oxide-montmorillonite hybrid influences diarrhea,intestinal mucosal integrity,and digestive enzyme activity in weaned pigs[J].Biological Trace Element Research,2012,149(2):190-196.

[16] QUINTEIRO-FILHO W M,RODRIGUES M V,RIBEIRO A,et al.Acute heat stress impairs performance parameters and induces mild intestinal enteritis in broiler chickens:role of acute hypothalamic-pituitary-adrenal axis activation[J].Journal of Animal Science,2012,90:1986-1994.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References