Short Communications

Enteromorpha prolifera Supplemental Level: Effects on Laying Performance, Egg Quality, Immune Function and Microflora in Feces of Laying Hens

  • WANG Shubai ,
  • JIA Yuhui ,
  • WANG Lihua ,
  • ZHU Fenghua ,
  • LIN Yingting
Expand
  • College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China

Received date: 2012-12-24

  Online published: 2013-05-15

Abstract

Effects of different supplemental levels of Enteromorpha prolifera on laying performance, egg quality, immune function, and microflora in feces of laying hens were studied in the present experiment. A total of ninety-six 42-week-old Highland brown laying hens with similar laying rate were randomly divided into 4 groups with 8 replicates per group and 6 birds per replicate. Hens were fed diets supplemented with 0 (control group), 1% (group 1), 2% (group 2) and 3% Enteromorpha prolifera meal (group 3). The experiment lasted for 28 days. The results showed as follows: dietary supplementation of Enteromorpha prolifera did not have significant effects on laying rate and average daily feed intake (P>0.05), but Enteromorpha prolifera significantly increased average egg weight (P<0.05), and significantly decreased feed:egg (P<0.05). Shell thickness of group 3 was significantly higher than that of control group. Shell calcium content and yolk color of the groups supplemented with Enteromorpha prolifera were significantly improved compared with control group (P<0.05), but yolk cholesterol content was significantly reduced (P<0.05). Hens fed Enteromorpha prolifera-supplemented diets had higher serum antibody titers of Newcastle Disease and lymphocyte transformation rate of blood, and there were significant difference between groups 2 and 3 and control group (P<0.05). In groups 2 and 3, the number of Escherichia coli in feces was significantly reduced (P<0.01 and P<0.05), while the number of Bifida bacterium in feces was significantly increased compared with control group (P<0.05 and P<0.01); in groups 1 and 2, the number of Lactobacillus bacterium was significantly increased compared with control group (P<0.01 and P<0.05). In conclusion, the supplementation of Enteromorpha prolifera up to 1%-3% can improve laying performance, egg quality, immune function and microflora in feces of laying hens in different degrees.

Cite this article

WANG Shubai , JIA Yuhui , WANG Lihua , ZHU Fenghua , LIN Yingting . Enteromorpha prolifera Supplemental Level: Effects on Laying Performance, Egg Quality, Immune Function and Microflora in Feces of Laying Hens[J]. Chinese Journal of Animal Nutrition, 2013 , 25(6) : 1346 -1352 . DOI: 10.3969/j.issn.1006-267x.2013.06.028

References

[1] 林文庭.浅论浒苔的开发与利用[J].中国食物与营养,2007(9):24-25.

[2] 高福成.新型海洋食品[M].北京:轻工出版社,1999:65-69.

[3] SEKINE K,OHTA J,ONISHI M,et al.Analysis of antitumor properties of effect or cells stimulated with a cell wall reparation (WPG) of Bifidobacterium infantis[J].Biological & Pharmaceutical Bulletin,1995,18(1):148-153.  

[4] 高和坤,姜锦鹏,柳丽,等.野生海藻粉作蛋鸡饲料添加剂的研究[J].淮海工学院学报,1999,8(2):51-53.

[5] 谷海源,刘永刚,舒子贞.海藻作饲料添加剂饲喂产蛋鸡的效果[J].饲料研究,1998(9):1-2.

[6] 刘纯仁.海藻饲料添加剂[J].中国饲料,1996(7):34-35.

[7] 魏尊,谷子林,李秋凤,等.饲料中添加海藻粉对蛋品质的影晌[J].饲料研究,2005(6):4-26.

[8] 赵军,王利华,朱风华,等.日粮中不同添加水平浒苔粉对蛋鸡生产性能及蛋品质的影响[J].中国饲料,2010(18):16-18.

[9] 赵军,林英庭,孙建凤,等.日粮中不同浒苔添加水平对蛋鸡营养物质表观消化率和盲肠微生物区系的影响[J].中国饲料,2010(23):15-17.

[10] 赵军,林英庭,孙建凤,等.饲粮中不同水平浒苔对蛋鸡蛋黄品质、抗氧化能力和血清生化指标的影响[J].动物营养学报,2011,23(3):452-458.

[11] 林英庭,朱风华,徐坤,等.青岛海域浒苔营养成分分析与评价[J].饲料工业,2009,30(3):46-49.

[12] 马建明,龚文杰,邬晨阳,等.直接皂化-硫酸铁铵比色法快速测定鸡蛋中胆固醇[J].中国卫生监测杂志,2001,11(3):12-15.

[13] 姚火春.兽医微生物学实验指导[M].2版.北京:中国农业出版社,2002:105-107.

[14] 林清华.免疫学实验[M].武汉:武汉大学出版社,1996:154-156.

[15] 黄国清,谢伟,王博.牛至油对肉鸡生产性能和肠道微生物菌群的影响[J].中国兽医杂志,2008,44(11):69-70.

[16] 石学连.浒苔多糖的化学组分及生物活性的初步研究[D].硕士学位论文.青岛:中国科学院海洋研究所,2009.

[17] 徐大伦.浒苔主要化学组分的分析及多糖活性的研究[D].硕士学位论文.青岛:中国海洋大学,2004.

[18] BRATOVA K,GANOVSKI K H.Effect of Black Sea algae on chicken egg production and on chick embyro development[J].Veterinarno-Meditsinski Nauki,1982,19(8):99-105.

[19] 曲湘勇,中岛隆.天然着色剂提高蛋黄色泽度的比较研究[J].中国畜牧杂志,1999(2):29-31.

[20] MCNAUGHTON J L.Effect of dietary fiber on egg yolk,liver and plasma cholesterol concentrations of the laying hen[J].The Journal of Nutrition,1978:108(11):1842-1848.

[21] 景建武,姚玉妮.海藻在动物生产中的应用研究进展[J].辽宁农业科学,2012(5):54-56.

[22] 王怀禹.海藻在畜禽养殖业中的应用[J].养殖与饲料,2009(4):23-25.

[23] 徐大伦,黄晓春,欧昌荣,等.浒苔多糖对非特异性免疫功能的体外实验研究[J].食品科学,2005(10):233-235.

[24] 阴天榜,刘兴友.家禽免疫学[M].北京:中国农业出版社,1999:203-207.

[25] 吕琴,姜乃澄.食用海藻对肠道菌群及肠道微环境影响的研究[J].东海海洋,2002(2):11-15.
Outlines

/