This experiment was conducted to investigate the effects of dietary vitamin E supplemental level on growth performance, blood and muscle physiochemical indexes of Megalobrama amblycephala. A total of 360 healthy Megalobrama amblycephala with an average body weight of (45.00?1.50) g were randomly divided into 6 groups with 4 replicates per group and 15 fish per replicate. Six isonitrogenous and isoenergentic experimental diets were formulated by supplementing 0, 12.5, 25.0, 50.0, 100.0 and 200.0 mg/kg vitamin E into the basal diet. Each diet was fed one of six groups for 90 days. The results showed as follows: 1) weight gain rate (WGR), specific growth rate (SGR) in the groups supplemented with 12.5, 25.0, 50.0, 100.0 and 200.0 mg/kg vitamin E and feeding rate in the groups supplemented with 25.0, 50.0, 100.0 and 200.0 mg/kg vitamin E were significantly higher than those in the control group (P<0.05). 2) Compared with the control group, plasma triacyglycerol (TG) content, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities in the groups supplemented with 12.5, 25.0, 50.0, 100.0 and 200.0 mg/kg vitamin E, plasma total cholesterol (TC) content in the groups supplemented with 12.5, 25.0, 50.0 and 100.0 mg/kg vitamin E, and plasma high density lipoprotein cholesterol (HDL-C) content in the groups supplemented with 12.5, 25.0 and 50.0 mg/kg vitamin E were significantly decreased (P<0.05); meanwhile, plasma alkaline phosphatase (ALP) activity in the group supplemented with 100 mg/kg vitamin E was significantly higher than that in the control group (P<0.05). 3) Muscle crude protein content was significantly increased in the groups supplemented with 25.0, 50.0, 100.0 and 200.0 mg/kg vitamin E compared with the control group (P<0.05). Muscle cooking meat rate in the groups supplemented with 12.5, 25.0, 50.0, 100.0 and 200.0 mg/kg vitamin E was significantly higher than that in the control group (P<0.05), while muscle drip loss rate in the groups supplemented with 25.0, 50.0 and 100.0 mg/kg vitamin E was significantly lower than that in the control group (P<0.05). These results suggest that the supplementation of 25.0 to 200.0 mg/kg vitamin E can enhance the growth and meat quality of Megalobrama amblycephala to some extent. Based on the WGR with regression equation analyses, the optimum vitamin E supplemental level in the diet is estimated to be 138.5 mg/kg for Megalobrama amblycephala.
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