The full-length cDNAs of the key enzymes of gluconeogenesis pathway, including phosphoenolpyruvate carboxykinase (PEPCK-C and PEPCK-M), fructose-1,6-bisphosphatase (FBPase) and glucose-6-phosphatase (G6Pase) were cloned from Siberian sturgeon (Acipenser baerii) liver using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE) methods. The results showed as follows: the full-length PEPCK-C cDNA of Siberian sturgeon (GenBank accession No. JQ995143) was 2 598 bp, consisting of an open reading frame (ORF) of 1 869 bp encoding a polypeptide of 622 amino acids with a theoretical molecular weight of 69.62 ku, and shared 77.3% to 80.5% similarity with other PEPCK-C genes. The full-length PEPCK-M cDNA of Siberian sturgeon (GenBank accession No. JQ995142) was 3 277 bp, consisting of an ORF of 1 935 bp encoding a polypeptide of 644 amino acids with a theoretical molecular weight of 71.11 ku, and shared 64.6% to 78.3% similarity with other PEPCK-M genes. The full-length FBPase cDNA of Siberian sturgeon (GenBank accession No. JF834908) was 1 372 bp, consisting of an ORF of 1 017 bp encoding a polypeptide of 338 amino acids with a theoretical molecular weight of 36.60 ku, and shared 73.4% to 88.7% similarity with other FBPase genes. The full-length G6Pase cDNA of Siberian sturgeon (GenBank accession No. JF834907) was 2 625 bp, consisting of an ORF of 1 080 bp encoding a polypeptide of 359 amino acids with a theoretical molecular weight of 40.62 ku, and shared 67.2% to 72.7% similarity with other G6Pase genes. The results of this study will provide a scientific basis for further study to explain the difference in the ability of dietary carbohydrate utilization and regulatory mechanism of gluconeogenesis in Siberian sturgeon compared with mammalian and carnivorous teleost.
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