Effects of Incubation Time on Casein Gene Expressions in Bovine Mammary Epithelial Cells in Three-Dimensional Culture

WANG Xiumei; HOU Xianzhi; AO Changjin; GAO Min; KAO Guilan; GAO Aiwu; LAN Rubing; TA Na

doi:10.3969/j.issn.1006-267x.2013.07.017

Chinese Journal of Animal Nutrition >

2013 , Vol. 25 >Issue 7: 1526 - 1533

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.07.017

Experimental Methods

Effects of Incubation Time on Casein Gene Expressions in Bovine Mammary Epithelial Cells in Three-Dimensional Culture

WANG Xiumei ,
HOU Xianzhi ,
AO Changjin ,
GAO Min ,
KAO Guilan ,
GAO Aiwu ,
LAN Rubing ,
TA Na

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1. College of Animal Science and Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Inner Mongolia Academy of Agriculture and Animal Husbandry, Hohhot 010010, China;
3. College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2013-01-22

Online published: 2013-06-17

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Abstract

This study was conducted to investigate the effects of incubation time on casein gene expressions in bovine mammary epithelial cells (BMECs) in three-dimensional culture. Mammary parenchymal tissue was obtained from three healthy Holstein dairy cows aged from 3 to 5 years. The first generation of BMECs was purified and cultured in three-dimensional culture for 3, 5, 7 and 9 days, respectively. The expression levels of αs1-casein, β-casein and κ-casein genes in BMECs in three-dimensional culture were detected. The results showed that the expression levels of αs1-casein and κ-casein genes in BMECs in three-dimensional culture for 5 days were significantly higher than those for 3, 7 and 9 days (P<0.01). The expression level of β-casein gene in BMECs in three-dimensional culture for 5 days was higher than that for 3 (P<0.01), 7 (P<0.01) and 9 days (P>0.05).The results indicate that the expression levels of αs1-casein, β-casein and κ-casein genes in BMECs are affected by incubation time in three-dimensional culture, and the optimal culture time is 5 days.

Key words： bovine mammary epithelial cells; three-dimensional culture; casein; gene expression; incubation time

Cite this article

WANG Xiumei , HOU Xianzhi , AO Changjin , GAO Min , KAO Guilan , GAO Aiwu , LAN Rubing , TA Na . Effects of Incubation Time on Casein Gene Expressions in Bovine Mammary Epithelial Cells in Three-Dimensional Culture[J]. Chinese Journal of Animal Nutrition, 2013 , 25(7) : 1526 -1533 . DOI: 10.3969/j.issn.1006-267x.2013.07.017

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