The aim of this study was to evaluate the effects of dilution rate on fermentation in a new type of liquid-solid-vapor diffluent rumen simulation system. A single factor completely random design was introduced to this experiment, four treatments were set, and dilution rates were 6%/h, 8%/h, 10%/h and 12%/h, respectively. The results showed that with the increase of dilution rate, fermentation liquid pH was significantly increased (P<0.01), but ammonia nitrogen concentration was significantly decreased (P<0.01). The concentrations of total volatile fatty acid and valeric acid were significantly decreased with the increase of dilution rate from 6%/h to 10%/h (P<0.01). Protozoa number was increased by 6.91% in 6%/h dilution rate treatment than that in 8%/h dilution rate treatment (P<0.01), but there was a significant descent (5.18%) for protozoa number when dilution rate was increased from 8%/h to 12%/h (P<0.01). With dilution rate increased from 6%/h to 10%/h, xylanase activity (P< 0.01) and microprotein production (P< 0.05) were significantly decreased, whereas crude protein in vitro disappearance rate was significantly increased (P<0.01). Neutral detergent fiber in vitro disappearance rate in treatments of 10%/h and 12%/h dilution rates was significantly higher than that in the other two treatments (P<0.05). In conclusion, dilution rate has impacts on fermentation in the new type of liquid-solid-vapor diffluent rumen simulation system, when the dilution rate is 8%/h, protozoa number, cellulase activities, and ruminal fermentation parameters are more beneficial for fermentation. Therefor, 8%/h dilution rate can be used as the optimal dilution rate for the system.
BAO Wanhua
,
WANG Jiaqi
,
BU Dengpan
,
JIANG Yahui
,
JIN Enwang
,
LUO Qiujiang
. Dilution Rate Affects Fermentation in a New Type of Liquid-Solid-Vapor Diffluent Rumen Simulation System[J]. Chinese Journal of Animal Nutrition, 2013
, 25(7)
: 1534
-1540
.
DOI: 10.3969/j.issn.1006-267x.2013.07.018
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