This experiment was conducted to investigate the effects of mannase supplementation on growth performance, digestion and metabolism, and non-specific immunity of tilapia (GIFT, Oreochromis niloticus). Three isonitrogenous (crude protein level was 33.5%) and isoenergetic (gross energy was 15.7 MJ/kg) experimental diets were foumulated by supplementing with 0 (control), 500 and 1 000 U/kg mannase, respectively. Each diet was randomly fed one group of fish with an initial average weight of (5.80±0.01) g for 8 weeks. Each group had 3 replicates with 30 fish per replicate. The results showed as follows: the final body weight, specific growth rate (SGR) and protein efficiency ratio (PER) of tilapia in the 1 000 U/kg group were significantly higher than those in the other groups (P＜0.05). No significant differences were found in feed conversation ratio (FCR), feed intake (FI) and survival rate (SR) among all groups (P＞0.05). With the increase of mannase supplemental level, the amylase, trypsase and Na+,K+-ATPase activities in intestine of tilapia were significantly increased (P＜0.05). The aspertate aminotransferase (AST) activity and high-density lipoprotein cholesterol (HDL-C) content in blood of tilapia in the 1 000 U/kg group were significantly lower than those in the control group and 500 U/kg group (P<0.05), but the total cholesterol (TC) and very low density lipoprotein cholesterol (VLDL-C) contents were significantly higher than those in the control group and 500 U/kg group (P<0.05). There were no significant differences in alanine aminotransferase (ALT) activity, total triglyceride (TG) content, low density lipoprotein cholesterol (LDL-C) content and white blood cell (WBC) count in blood, and total superoxide dismutase (T-SOD) activity in serum among all groups (P＞0.05). The percentages of neutrophils and moncytes in blood and the activities of alkaline phosphatase (AKP) and catalase (CAT) in serum in the 500 and 1 000 U/kg groups were significantly higher than those in the control group (P＜0.05), but the percentage of lymphocyte in blood was significantly lower than that in the control group (P＜0.05). These results indicate that a suitable level of mannase supplementation can improve the ability of digestion and metabolism, enhance the non-specific immunity, and then promote the growth of tilapia.
MAO Shuhong
,
LIN Xin
,
YANG Yang
,
LIN Shimei
,
LUO Lin
,
LI Binbin
. Effects of Mannase on Growth Performance, Digestion and Metabolism, and Non-Specific Immunity of Tilapia (GIFT, Oreochromis niloticus)[J]. Chinese Journal of Animal Nutrition, 2013
, 25(7)
: 1641
-1647
.
DOI: 10.3969/j.issn.1006-267x.2013.07.031
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