This experiment was conducted to study growth characteristics of lignin-degrading fungi in sampling points of different areas under the condition of soil components, and to lay the foundation for the screening advantage bacteria of degrading straw lignin. The growth of lignin-degrading fungi and properties of lignin degradation enzyme in different soils were identified by measuring the soil composition in 5 sampling points of Yangzhou, Nanjing, Linyi, Weifang and Lianyungang, and using the selective medium and the coloration reaction of PDA-RB plate and PDA-Bavendammplate. Finally, advantage straines were chosen according to the growth characteristics of strains in different regional sampling points. The results showed that lignin-degrading fungi density in soil sampling point of Yangzhou was significantly higher than that in the other four sampling points (P<0.01), and the property of lignin-degrading fungi in producing enzyme was also superior to the other sampling points. Therefore, the lignin-degrading fungi density in soil of these five sampling points is greatly different, and it is in a strong positive correlation with the soil organic carbon content (r=0.84, P<0.01).
BIE Chunyu
,
JIANG Tao
,
JIANG Xiaoyun
,
ZHANG Shiyuan
. Screening and Comparison of Growth Characteristics of Lignin-Degradating Advantage Strains in Soil of Different Regional Sampling Points[J]. Chinese Journal of Animal Nutrition, 2013
, 25(7)
: 1656
-1662
.
DOI: 10.3969/j.issn.1006-267x.2013.07.033
[1] 谢光辉,王晓玉,任兰天.中国作物秸秆资源评估研究现状[J].生物工程学报,2010,26(7):855-863.
[2] 陶用珍,官映亭.木质素的化学结构及其应用[J].纤维素科学与技术,2003,11(1):42-50.
[3] TUOR U,WINTERHALTER K,FIECHTER A.Enzymes of white-rot fungi involved in lignin degradation and ecological determinants for wood decay[J].Journal of Biotechnology,1995,41(1):1-17.
[4] AKIN D E,MORRISON Ⅲ W H,RIGSBY L L,et al.Biological delignification of plant components by the white rot fungi Ceriporiopsis subvermispor and Cyathus stercoreus[J].Animal Feed Science and Technology,1996,63(1/2/3/4):1-17.
[5] 张力,邵喜霞,韩大勇.白腐真菌木质素降解酶系研究进展[J].畜牧与饲料科学,2009,30(1):35-37.
[6] ROBINSON T,CHANDRAN B,NIGAM P.Studies on the production of enzymes by white-rot fungi for the decolourisation of textile dyes[J].Enzyme and Microbial Technology,2001,29(8/9):575-579.
[7] 徐晓峰,何北海,徐丽丽,等.白腐菌的筛选及对松木片木质素和树脂的脱除[J].华南理工大学学报:自然科学版,2007,35(4):105-111.
[8] 吕镇梅,李硕文,同帜.白腐菌降解造纸黑液中木质素的研究[J].西北纺织工学院学报,2000,14(4):406-411.
[9] 邢来君.普通真菌学[M].北京:高等教育出版社,1999:270-280.
[10] 吴坤,朱显峰,张世敏,等.杂色云芝产漆酶的发酵条件研究[J].菌物系统,2001,20(2):207-213.
[11] 杨刚,何寻阳.不同植被类型对土壤微生物量碳氮及土壤呼吸的影响[J].土壤通报,2008,39(1):189-191.
[12] MADHAVI S,LELE S.Enhanced production of laccase using a new isolate of white rot fungus WR-1[J].Process Biochemistry,2006,41(3):581-588.
[13] LEE K,MOON S H.Electroenzymatic oxidation of veratryl alcohol by lignin peroxidase[J].Journal of Biotechnology,2003,102(3):261-268.
[14] NISHIDA T,TSUTSUMI Y,LIYOSHI Y,et al.Polyethylene degradation by lignin-degrading fungi and manganese peroxidase[J].The Japan Wood Research Society,1998,44(3):222-229.
[15] MOSAI S,WOLFAARDT J F,PRIOR B A.Evaluation of selected white-rot fungi for biosulfite pulping[J].Bioresource Technology,1999,68(1):89-93.
[16] RODRGUEZ A,PERESTELO F,CARNICERO A.Degradation of natural lignins and lignocellulosic substrates by soil-inhabiting fungi imperfecti[J].FEMS Microbiology Ecology,1996,21(3):213-219.
[17] 池玉杰,伊洪伟.木材白腐菌分解木质素的酶系统——锰过氧化物酶、漆酶和木质素过氧化物酶催化分解木质素的机制[J].菌物学报,2007,26(1):153-160.
[18] 关艳丽,李莉.1株产漆酶白腐真菌的筛选和鉴定[J].微生物学杂志,2010,30(3):74-77.
