This study was conducted to investigate the effects of 25-OH-D₃ and 1α-OH-D₃ replacing vitamin D₃ on growth performance, serum biochemical indices, tibia development and nutrient digestibility of yellow-feathered broilers aged from 42 to 63 days. A total of 2 400 male yellow-feathered broilers aged 42 days were randomly divided into 8 groups with 5 replicates per group and 60 chickens in each replicate. Treatment 1 (control group) was fed a basal diet, in which the contents of vitamin D₃, calcium, phosphorus were 2 780 IU/kg, 0.8%, and 0.55%, respectively. For treatments 2 (low phosphorus and calcium control group) to 8, the contents of vitamin D₃, calcium, phosphorus were 0 IU/kg, 0.7%, 0.45%, respectively. For treatments 3 to 5, the contents of 25-OH-D₃ were 50, 70 and 90 μg/kg, respectively. For treatments 6 to 8, the contents of 1α-OH-D₃ were 2.5, 5.0 and 10.0 μg/kg, respectively. The experiment lasted for 21 days. The results showed as follows: 1) compared with low calcium and phosphorus control group, except that 50 μg/kg 25-OH-D₃ did not affect average daily feed intake (P>0.05), the supplementation of different levels of 25-OH-D₃ or 10.0 μg/kg 1α-OH-D₃ significantly improved the growth performance of yellow-feathered broilers (P<0.05); the supplementation of 70 and 90 μg/kg 25-OH-D₃ or 2.5, 5.0 and 10.0 μg/kg 1α-OH-D₃ significantly improved calcium apparent and true digestibility (P<0.05), and the supplementation of 5.0 and 10.0 μg/kg 1α-OH-D₃ significantly improved phosphorus apparent and true digestibility (P<0.05); the supplementation of 50, 70 and 90 μg/kg 25-OH-D₃ or 10.0 μg/kg 1α-OH-D₃ significantly improved serum phosphorus content and decreased serum the ratio of calcium to phosphorus (Ca/P) (P<0.05), and the supplementation of 90 μg/kg 25-OH-D₃ or 10.0 μg/kg 1α-OH-D₃ significantly decreased serum tartrate resistant acid phosphatase (TRAP) activity (P<0.05), while there was no significant difference in serum alkaline phosphatase (ALP) activity of yellow-feathered broilers among all treatments (P>0.05); the supplementation of 90 μg/kg 25-OH-D₃ or 10.0 μg/kg 1α-OH-D₃ significantly improved ash weight, Ca/P, fresh weight, defatted weigh, defatted weigh index, length, broken strength and bone density of tibia (P<0.05), but had no influence on ash ratio, calcium content and fresh weight index of tibia (P>0.05). 2) Compared with the normal calcium and phosphorus control group, the supplementation of 70 and 90 μg/kg 25-OH-D₃ and 2.5, 5.0 and 10.0 μg/kg 1α-OH-D₃ significantly increased calcium apparent digestibility (P<0.05), and the supplementation of 10.0 μg/kg 1α-OH-D₃ significantly increased phosphorus apparent and true digestibility (P<0.05); the supplementation of 90 μg/kg 25-OH-D₃ or 10.0 μg/kg 1α-OH-D₃ significantly increased serum phosphorus content and Ca/P (P<0.05), and decreased tibia mineralization (P<0.05), but had no significant effects on growth performance, serum TRAP and ALP activities and tibia development indices expect for mineralization and ash weight of yellow-feathered broilers (P>0.05). These results indicate that reducing calcium and phosphate levels in diets and adding 25-OH-D₃ or 1α-OH-D₃ replacing vitamin D₃ can effectively improve the digestibility of phosphorus and calcium, growth performance and tibia development of yellow-feathered broilers. Of all the treatments, the addition of 90 μg/kg 25-OH-D₃ or 10.0 μg/kg 1α-OH-D₃ has the best results in yellow-feathered broilers.