Effects of Different Amino Acid Patterns on Casein Synthesis in Mammary Epithelial Cells of Dairy Cows

ZHANG Xingfu; DU Ruiping; AO Changjin; GAO Min; LU Dexun

doi:10.3969/j.issn.1006-267x.2013.08.013

Chinese Journal of Animal Nutrition >

2013 , Vol. 25 >Issue 8: 1762 - 1768

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.08.013

Ruminants and Herbivorous Animal Nutrition

Effects of Different Amino Acid Patterns on Casein Synthesis in Mammary Epithelial Cells of Dairy Cows

ZHANG Xingfu ,
DU Ruiping ,
AO Changjin ,
GAO Min ,
LU Dexun

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1. College of Animal Science, Inner Mongolia Agricultural University, Huhhot 010018, China;
2. Animal Nutrition Institute, Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Huhhot 010031, China

Received date: 2012-02-18

Online published: 2013-07-15

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Abstract

The objective of this study was to determine whether different amino acid (AA) patterns could affect casein synthesis in mammary epithelial cells of dairy cows. A completely random design was used. Four AA patterns were as follows: blood in low protein diet AA pattern (LPBP group), milk protein AA pattern (MP group), 80% casein+20% lactoalbumin AA pattern (CLP group) and casein AA pattern (CP group). Each group had 3 replicates, and all experiments were repeated 3 times. The mRNA expression levels of α_s1-casein (CSN1S1) and κ-casein (CSN3) genes and the content of α_s-casein were determined by real-time quantitative PCR (RT-qPCR) method and ELISA, respectively. The results showed that different AA patterns could significantly affect the mRNA expression levels of CSN1S1 and CSN3 genes (P<0.05). The mRNA expression levels of CSN1S1 and CSN3 gens in MP group were significantly higher than those in LPBP group (P<0.05 and P<0.01, respectively). Compared with the LPBP group, the mRNA expression levels of CSN1S1 and CSN3 genes in MP group were increased by 1.70-fold and 2.12-fold,respectively. The synthesis content of α_s-casein in MP group was significantly higher than that in CLP and CP groups (P<0.05), and was significantly higher than that in LPBP group (P<0.01). In conclusion, different AA patterns play a very important role in milk protein synthesis in mammary epithelial cells of dairy cows, and the milk protein AA pattern may be an appropriate AA pattern.

Key words： mammary epithelial cells; amino acid pattern; gene expression; casein

Cite this article

ZHANG Xingfu , DU Ruiping , AO Changjin , GAO Min , LU Dexun . Effects of Different Amino Acid Patterns on Casein Synthesis in Mammary Epithelial Cells of Dairy Cows[J]. Chinese Journal of Animal Nutrition, 2013 , 25(8) : 1762 -1768 . DOI: 10.3969/j.issn.1006-267x.2013.08.013

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