Duodenal Infusion of Leucine: Effects on Pancreatic Amylase Secretion of Dairy Cows

LIU Ye; LIU Kai; XU Ming; LYU Changrong; CAO Yangchun; YAO Junhu

doi:10.3969/j.issn.1006-267x.2013.08.016

Chinese Journal of Animal Nutrition >

2013 , Vol. 25 >Issue 8: 1785 - 1790

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.08.016

Ruminants and Herbivorous Animal Nutrition

Duodenal Infusion of Leucine: Effects on Pancreatic Amylase Secretion of Dairy Cows

LIU Ye ,
LIU Kai ,
XU Ming ,
LYU Changrong ,
CAO Yangchun ,
YAO Junhu

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1. College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China;
2. College of Animal Science, Inner Mongolia Agricultural University, Huhhot 010080, China;
3. College of Veterinary Medicine, Northwest A & F University, Yangling 712100, China

Received date: 2013-03-25

Online published: 2013-07-15

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Abstract

This experiment was conducted to evaluate the effects of duodenal leucine infusion on pancreatic α-amylase secretion of dairy cows. Four intravenously cannulated yearling Holstein cows [(215±7) kg] with pancreatic pouch-duodenal reentrant cannulas, duodenal catheters and jugular catheters were used in a 4×4 Latin square with duodenal leucine infusions [0, 29.5, 59.1 and 88.6 μmol/(kg·h)]. The results showed as follows: plasma insulin and cholecystokinin concentrations were increased with leucine infusion level increasing (P<0.05), and pancreatic α-amylase production rate (U/h) was increased first and then decreased with leucine infusion level increasing (P<0.01), in which the greatest value was observed at 29.5 μmol/(kg·h). Duodenal leucine infusion could also affect α-amylase concentration (U/L, P<0.01; U/g prot, P<0.01). These data indicate that leucine can regulate pancreatic α-amylase secretion by stimulating the release of insulin and cholecystokinin of young daily cows, and there is a dose-effect relationship between them.

Key words： leucine; duodenal infusion; amylase; insulin; cholecystokinin

Cite this article

LIU Ye , LIU Kai , XU Ming , LYU Changrong , CAO Yangchun , YAO Junhu . Duodenal Infusion of Leucine: Effects on Pancreatic Amylase Secretion of Dairy Cows[J]. Chinese Journal of Animal Nutrition, 2013 , 25(8) : 1785 -1790 . DOI: 10.3969/j.issn.1006-267x.2013.08.016

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