Effects of Levels of Ammoniated Rice Straw Included in Rations on Rumen Methane and Volatile Fatty Acids Production in in Vitro Incubation

WANG Manhong; ZHAO Guangyong

doi:10.3969/j.issn.1006-267x.2013.08.017

2013 , Vol. 25 >Issue 8: 1775 - 1784

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.08.017

Ruminants and Herbivorous Animal Nutrition

Effects of Levels of Ammoniated Rice Straw Included in Rations on Rumen Methane and Volatile Fatty Acids Production in in Vitro Incubation

WANG Manhong ,
ZHAO Guangyong

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College of Animal Science and Technology, China Agricultural University, State Key Laboratory of Animal Nutrition, Beijing 100193, China

Received date: 2013-02-18

Online published: 2013-07-15

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Abstract

The objective of this experiment was to study the effects of different proportions of untreated and ammoniated rice straw in mixed rations on methane and volatile fatty acids (VFA) production. Two Simmental cattle fitted with rumen cannulas were used as the donors of rumen fluid. Three levels of urea, i.e. 0% (untreated), 4% and 6% were used for ammoniation treatment of rice straw, respectively. The untreated and ammoniated rice straws were mixed with concentrate mixture at the ratios of 0:100, 20:80, 40:60, 60:40, 80:20 and 100:0, respectively, as experimental treatments. The gas production technique of Menke et al. (1979) was used for in vitro fermentation and the measurement of 48 h VFA, methane and total gas production. The results showed that 48 h total VFA (TVFA) production of the groups with 4% urea ammoniated rice straw and 6% urea ammoniated rice straw was significantly higher than that of the group with non-ammoniated rice straw when concentrate/roughage ratio was 0:100 (P<0.05). The production of acetate, propionate and TVFA was significantly increased with the increase of concentrate/roughage ratio in the ration (P<0.05), and acetate/propionate and acetate/TVFA were gradually reduced (P<0.05). The 48 h methane production of the groups with 4% urea ammoniated rice straw and 6% urea ammoniated rice straw was significantly higher than that of the group with non-ammoniated rice straw when concentrate/roughage ratio was 40:60 (P<0.05), but 48 h total gas production of the group with 4% urea ammoniated rice straw was significantly higher than that of the group with non-ammoniated rice straw (P<0.05), and was higher than that of the group with 6% urea ammoniated rice straw to some extent (P>0.05). The 48 h methane and total gas production was significantly increased with the increase of concentrate/roughage ratio in the ration (P<0.05). The best associative effect of 48 h total gas and methane production was found in the group with 4% urea ammoniated rice straw and concentrate/roughage ratio of 40:60. The 48 h total gas production in each group had a positive associative effect, but CH₄/TVFA in the group with 4% urea ammoniated rice straw had a negative associative effect. This study supports that methane production is affected by both concentrate/roughage ratio and quality of forage in the ration. The 48 h total gas and the methane production are highest, but CH₄/TVFA is the lowest when the ratio of concentrate/4% urea ammoniated rice straw is 40:60.

Key words： rice straw; ammoniation; rumen; methane; volatile fatty acids

Cite this article

WANG Manhong , ZHAO Guangyong . Effects of Levels of Ammoniated Rice Straw Included in Rations on Rumen Methane and Volatile Fatty Acids Production in in Vitro Incubation[J]. Chinese Journal of Animal Nutrition, 2013 , 25(8) : 1775 -1784 . DOI: 10.3969/j.issn.1006-267x.2013.08.017

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