Glutaredoxin 1 and Thioredoxin 1: Gene Expression Characteristics in Different Tissues and Effects of Vitamin E on Gene Expressions in Oxidative Stress Cells of Yunnan Gaoligongshan Pigs

AN Qingcong; ZHANG Chunyong; LI Meiquan; CHEN Kelin; GUO Rongfu

doi:10.3969/j.issn.1006-267x.2013.08.020

2013 , Vol. 25 >Issue 8: 1825 - 1835

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.08.020

Molecular Nutrition

Glutaredoxin 1 and Thioredoxin 1: Gene Expression Characteristics in Different Tissues and Effects of Vitamin E on Gene Expressions in Oxidative Stress Cells of Yunnan Gaoligongshan Pigs

AN Qingcong ,
ZHANG Chunyong ,
LI Meiquan ,
CHEN Kelin ,
GUO Rongfu

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Yunnan Key Laboratory of Animal Nutrition and Feeds, Yunnan Agricultural University, Kunming 650201, China

Received date: 2013-02-01

Online published: 2013-07-15

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Abstract

This experiment was conducted to study glutaredoxin 1 (GRX1) and Thioredoxin 1 (TRX1) gene expression characteristics in different tissues and investigate the regulation of vitamin E on the expressions of those two genes in oxidative stress cells of Yunnan Gaoligongshan pigs. The expressions of GRX1 and TRX1 genes in 11 tissues (hypophysis, hypothalamus, thyroid, thymus, pancreas, germen, duodenum, jejunum, ileum, skin and liver) were determined by the method of real time-quantitative PCR (RT-qPCR); pig fetal skin fibroblasts (PFSF) oxidative stress model was established induced by H₂O₂, and the regulation of vitamin E on GRX1 and TRX1 gene expressions in oxidative stress cells was explored. The results showed as follows: 1) GRX1 and TRX1 gene expressions were detected in all tissues, the highest expressional levels of GRX1 and TRX1 were in liver, and then skin and jejunum, and the lowest expressional levels was in pancreas and other endocrine glands; the expression level of GRX1 gene in thymus, germen, ileum and liver of sows was significantly higher than that of boars (P<0.05), and that in pancreas of sows was extremely higher than that of boars (P<0.01). The expression level of TRX1 gene in hypophysis, thymus, pancreas, germen and liver of sows was significantly higher than that of boars (P<0.05). 2) The results of cell culture indicated that TRX1 gene was over expressed in oxidative stress cells (P<0.05). Vitamin E regulated GRX1 and TRX1 gene expressions in those oxidative stress cells, and the optimal concentration of vitamin E was 40 μg/mL. In conclusions, the expressions of GRX1 and TRX1 genes of Gaoligongshan pigs have obvious tissue- and gender-specific manner; inducing those gene expressions might be an important way to relieve oxidative stress; the expressions of GRX1 and TRX1 genes and antioxidant ability can be improved by vitamin E supplementation at a suitable concentration.

Key words： GRX1; TRX1; gene expression; Gaoligongshan pigs; oxidative stress; vitamin E

Cite this article

AN Qingcong , ZHANG Chunyong , LI Meiquan , CHEN Kelin , GUO Rongfu . Glutaredoxin 1 and Thioredoxin 1: Gene Expression Characteristics in Different Tissues and Effects of Vitamin E on Gene Expressions in Oxidative Stress Cells of Yunnan Gaoligongshan Pigs[J]. Chinese Journal of Animal Nutrition, 2013 , 25(8) : 1825 -1835 . DOI: 10.3969/j.issn.1006-267x.2013.08.020

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