This experiment was conducted to study the effects of dietary corn distillers dried drains with solubles (DDGS) level on laying performance, egg quality, serum lipid indices and economic profit of Suqin green-shelled laying hens. Two hundred and forty 28-week-old Suqin green-shelled laying hens with a similar laying rate were assigned to 6 groups with 4 replicates in each group and 10 hens per replicate. Experimental diets contained 0 (control group), 1%, 3%, 5%, 7% and 9% DDGS which consisted of 26.91% crude protein, 9.20 MJ/kg apparent metabolizable energy, 13.66% crude fat, 32.27% neutral detergent fiber, 9.58% acid detergent fiber, 0.12% calcium, and 0.66% phosphorus. The experiment lasted for 42 days. Results showed as follows: 1) compared with the control group, corn DDGS significantly increased yolk color (P<0.05), and Haugh unit was significantly increased when the level of dietary corn DDGS was or exceed 3% (P<0.05), but different corn DDGS levels did not affect laying performance and the other egg quality indices (P>0.05). The serum cholesterol content in 7% and 9% corn DDGS groups was significantly lower compared with the control group (P<0.05). 2) In the experiment, compared with the control group, the feed cost in 1%, 3%, 5%, 7% and 9% corn DDGS groups was reduced by 0.151, 0.211, 0.383, 0.478 and 0.495 RMB per bird, and the profit in 7% and 9% corn DDGS groups was increased by 0.170 and 0.933 RMB per bird. It is indicated that corn DDGS has no harmful effects on laying performance, egg quality and yolk cholesterol content, and 7% and 9% DDGS significantly increase the yolk color and Haugh unit, decrease serum CHOL content, and can effectively increase the profit.