Light and Transmission Electron Microscopical Observation of Ruminal Papillae of Dairy Cows Receiving Different Types of Diets

WENG Xiuxiu; ZHANG Yangdong; LI Fadi; ZHOU Lingyun; BU Dengpan

doi:10.3969/j.issn.1006-267x.2013.09.011

2013 , Vol. 25 >Issue 9: 1998 - 2004

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.09.011

Molecular Nutrition

Light and Transmission Electron Microscopical Observation of Ruminal Papillae of Dairy Cows Receiving Different Types of Diets

WENG Xiuxiu ,
ZHANG Yangdong ,
LI Fadi ,
ZHOU Lingyun ,
BU Dengpan

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1. State Key Laboratory of Ruminant Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China

Received date: 2013-03-02

Online published: 2013-08-18

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Abstract

The objective of this study was to determine the effects of different types of diets on ruminal papillae histomorphology of dairy cows. Ten primiparous and rumen-cannulated Holstein dairy cows averaging (127?13) d in milk were randomly assigned to 2 groups, and each group had 5 dairy cows. The dairy cows were fed two types of diets, one of them was formulated with corn straw as sole forage and high level concentrate (CS group) and the other was formulated with chinese wildrye+alfalfa hay+corn silage as mixed forage and low level concentrate (MF group). The experiment lasted for 63 days. At the end of this experiment, ruminal papillae were biopsied from the ventral sac and observed using light and transmission electron microscope. The results showed as follows: the width of papillae in CS group was significantly higher than that in MF group (P<0.01). The thickness of stratum granulosum, stratum spinosum, and stratum basale in MF group were higher than those in CS group, the thickness of stratum corneum was lower than that in CS group, but no significant differences were found between the two groups (P>0.05). Biopsied papillae exhibited a decline in cellular junctions and large spaces between cells of stratum granulosum, moderate sloughing of the stratum corneum, and an accelerated migratory state in all strata below the stratum corneum in CS group. These results suggest that feeding the diet formulated with corn straw and high level concentrate affects ruminal papillae morphological structure of dairy cows.

Key words： dietary types; ruminal papillae; histomorphology

Cite this article

WENG Xiuxiu , ZHANG Yangdong , LI Fadi , ZHOU Lingyun , BU Dengpan . Light and Transmission Electron Microscopical Observation of Ruminal Papillae of Dairy Cows Receiving Different Types of Diets[J]. Chinese Journal of Animal Nutrition, 2013 , 25(9) : 1998 -2004 . DOI: 10.3969/j.issn.1006-267x.2013.09.011

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