Different Roughage Combinations in Total Mixed Rations：Effects on Microbial Protein Concentration Variation in Rumen Fluid in 24 Hours and Its Flow in Small Intestine of Lactating Cows

ZHANG Zhihui; YANG Hongjian; REN Qingchang; JIN Xin; LI Shengli

doi:10.3969/j.issn.1006-267x.2013.09.012

2013 , Vol. 25 >Issue 9: 2005 - 2011

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.09.012

Ruminant Nutrition

Different Roughage Combinations in Total Mixed Rations：Effects on Microbial Protein Concentration Variation in Rumen Fluid in 24 Hours and Its Flow in Small Intestine of Lactating Cows

ZHANG Zhihui ,
YANG Hongjian ,
REN Qingchang ,
JIN Xin ,
LI Shengli

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State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China

Received date: 2013-03-02

Online published: 2013-08-18

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Abstract

This experiment was conducted to study the effects of different roughage combinations in total mixed ration (TMR) on microbial protein (MCP) concentration variation in rumen fluid in 24 hours and its flow in small intestine of lactating cows by the purine derivative (PD) method. Five 4-year-old Holstein cows with an average body weight of (543±45) kg, similar lactation period and average daily milk yield, fitted with permanent ruminal fistulas, were used in a 5×5 Latin square design. Roughage combinations in five TMRs (concentrate:forage= 61:39) were as follows: 100% corn stover (TMR1), 50% corn stover+50% ensiled corn stover (TMR2), 50% ensiled corn stover+50% Chinese wildrye hay (TMR3), 50% Chinese wildrye hay+50% whole corn silage (TMR4), and 34% Chinese wildrye hay+33% whole corn silage+33% alfalfa hay (TMR5). The results showed as follows: MCP concentration in rumen fluid did not significantly differ among different time points (sampled every 2 hours) (P>0.05), but significantly differ among different TMRs (P<0.05). The highest MCP concentration (1.217 mg/mL) occurred in TMR3, and it was significantly higher than that of TMR1 and TMR5 (P<0.05), but was not significantly different from that of TMR2 and TMR4 (P>0.05). Allantoin, uric acid and PD outputs in urine and microbial nitrogen (MN) and MCP amount flowed into small intestine gradually increased from TMR1 to TMR5, and the difference finally reached a significant level (P<0.05). Allantoin and uric acid outputs in PD output ranged from 83% to 92% and 8% to 17%, respectively. Allantoin (y=50.44x+35.58, R²=0.733), uric acid (y=27.22x-28.77, R²=0.734) and PD outputs (y=77.67x+6.811, R²=0.734) were linearly correlated with NFC/NDF in the TMRs. Consequently, PD output in urine and MCP amount flowed into small intestine in the TMR with a roughage combination of Chinese wildrye hay, whole corn silage and alfalfa hay are higher than those in the other TMRs.

Key words： roughage combination; lactating cows; purine derivative; microbial protein

Cite this article

ZHANG Zhihui , YANG Hongjian , REN Qingchang , JIN Xin , LI Shengli . Different Roughage Combinations in Total Mixed Rations：Effects on Microbial Protein Concentration Variation in Rumen Fluid in 24 Hours and Its Flow in Small Intestine of Lactating Cows[J]. Chinese Journal of Animal Nutrition, 2013 , 25(9) : 2005 -2011 . DOI: 10.3969/j.issn.1006-267x.2013.09.012

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