Molecular Nutrition

Bacterial Diversity in Rumen of Sika Deer (Cervus nippon) Based on 16S rRNA Sequences Analysis

  • LI Zhipeng ,
  • LIU Hanlu ,
  • CUI Xuezhe ,
  • JING Yi ,
  • BAO Kun ,
  • XU Chao ,
  • YANG Fuhe ,
  • LI Guangyu
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  • Jilin Provincial Key Labororary for Molecular Biology of Special Economic Animals, Insitute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, China

Received date: 2013-03-07

  Online published: 2013-08-18

Abstract

Bacteiral diversity in the rumen of sika deer feeding tannin-rich plants (oak leaves) was investigated. The extrated genomic DNA was used to amplify bacteria 16S rRNA gene. Bacterial communities in the rumen of sika deer were determined based on the 16S rRNA gene library. The results showed as follows: 1) a total of 107 non-chimeric 16S rRNA gene sequences were obtained. These sequences were assinged to 22 operational taxonomic units (OTUs) based on 97% sequence similarity. Ninety-one sequences (10 OTUs, 85% of total clones) showed ≥97% sequence similarity to known species, thirteen sequences (10 OTUs, 12.2% of total clones) had sequence similarity to known species in the range of 90% to 97%, and the remaining sequences displayed similarity less than 90%. Eighty-seven point nine percent sequences were similar to Prevotella spp.. 2) Phylogenetic analysis indicated that bacteria in the rumen of sika deer belonged to phyla Bacteriodetes and Firmicutes. It is concluded that Prevotella spp. is the dominant bacteria in rumen of sika deer feeding oak leaves containing high content of tannins. While, the lack of fibrolytic bactria in the rumen of sika deer may be the result of hight content of tannins in diets.

Cite this article

LI Zhipeng , LIU Hanlu , CUI Xuezhe , JING Yi , BAO Kun , XU Chao , YANG Fuhe , LI Guangyu . Bacterial Diversity in Rumen of Sika Deer (Cervus nippon) Based on 16S rRNA Sequences Analysis[J]. Chinese Journal of Animal Nutrition, 2013 , 25(9) : 2044 -2050 . DOI: 10.3969/j.issn.1006-267x.2013.09.017

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