Sensitivity and Confidence Limit of Empting-Force Feeding Method for Determining Apparent Metabolizable Energy of Diets in Rooster

WANG Meiqin; ZHAO Feng; JIA Gang; LIU Chengling; WANG Yumin; ZHANG Hongfu

doi:10.3969/j.issn.1006-267x.2013.09.019

2013 , Vol. 25 >Issue 9: 2059 - 2066

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.09.019

Experimental Methods

Sensitivity and Confidence Limit of Empting-Force Feeding Method for Determining Apparent Metabolizable Energy of Diets in Rooster

WANG Meiqin ,
ZHAO Feng ,
JIA Gang ,
LIU Chengling ,
WANG Yumin ,
ZHANG Hongfu

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1. Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, China;
2. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2013-03-12

Online published: 2013-08-18

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Abstract

The sensitivity and confidence limit of empting-force feeding method for determining the apparent metabolizable energy (AME) values of diets in rooster were investigated to make a reference for the national standard method of GB/T 26437—2010. In experiment 1, a 2×5 completely randomized design with 2 basal diets and 5 diluent levels (2%, 4%, 6%, 8% and 10% of peanut hull in the basal diets) was adopted to determine the response of determined AME values using empting-force feeding method to the calculated AME values. In experiment 2, according to the variances of the AME values of 10 diets in experiment 1 and 4 batches of blank samples, the confidence limit was determined for empting-force feeding method to differentiate the AME values between two samples. The results showed that in two groups of experimental diets composed of the basal diet and peanut hull, there was a significant linear effect of peanut hull content on the AME values of the diets (P<0.01), whereas the quadratic effects and lack-of-fit of linearity were not significant (P>0.05). A linear regression with a slope of 0.987 was observed between the independent variable of calculated AME values and dependent variable of measured AME values of the diets (P<0.01), indicating there was a high sensitivity of empting-force feeding method. There was no significant difference in the variances of the AME values of the 10 diets and the blank sample (P>0.05). According to the mean of standard deviation of dietary AME values, 0.27 MJ/kg DM of confidence limit was calculated for empting-force feeding method to differentiate the AME values between two samples. In conclusion, the sensitivity of empting force-feeding is satisfactory. The 0.27 MJ/kg DM of confidence limits is a critical parameter for the determination of significant difference among diets.

Key words： empting-force feeding method; rooster; apparent metabolizable energy; sensitivity; confidence limit

Cite this article

WANG Meiqin , ZHAO Feng , JIA Gang , LIU Chengling , WANG Yumin , ZHANG Hongfu . Sensitivity and Confidence Limit of Empting-Force Feeding Method for Determining Apparent Metabolizable Energy of Diets in Rooster[J]. Chinese Journal of Animal Nutrition, 2013 , 25(9) : 2059 -2066 . DOI: 10.3969/j.issn.1006-267x.2013.09.019

References

[1] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员.GB/T 26437—2010畜禽饲料有效性与安全性评价强饲法测定鸡饲料表观代谢能技术规程[S].北京:中国标准出版社,2010.

[2] DALE N M,FULLER H L.Repeatability of true metabolizable energy versus nitrogen corrected true metabolizable energy values[J].Poultry Science,1986,65:352-354.

[3] 夏伦志,吴东,钱坤,等.小麦型干酒糟及其可溶物在淮南麻黄鸡上能量及氨基酸代谢率的测定[J].动物营养学报,2013,25(2):364-371.

[4] BOURDILLON A,CARRE B,CONON L,et al.European reference method for the in vivo determination of metabolisable energy with adult cockerels:reproducibility,effect of food intake and comparison with individual laboratory methods[J].British Poultry Science,1990,31(3):557-565.

[5] BOURDILLON A,CARRE B,CONON L,et al.European reference method of in vivo determination of metabolisable energy in poultry:reproducibility,effect of age,comparison with predicted values[J].British Poultry Science,1990,31(3):566-576.

[6] 米宝民,赵峰,谭会泽,等.排空强饲法测定鸡饲料表观代谢能值变异程度及其来源[J].动物营养学报,2012,24(11):2172-2180.

[7] ALFONSO T H D,MANBECK H B,ROUSH W B.Partitioning of variance in true metabolizable energy determinations:an example using wheat data[J].Animal Feed Science and Technology,1999,80:29-41.

[8] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员.GB/T 6435—2006饲料中水分和其他挥发性物质含量的测定[S].北京:中国标准出版社,2006.

[9] KAPS M,LAMBERSON W R.Biostatistics for animal science[M].Cambridge:CABI Publishing,2004.

[10] BALTHROP J,BENEDIKT B,RICHARD A C,et al.Quality assurance for animal feed analysis laboratories[M].Rome:FAO Animal Production and Health Manual No.14,2011.

[11] SIBBALD I R.A test of the additivity of true metabolizable energy values of feedingstuffs[J].Poultry Science,1977,56:363-366.

[12] HONG D,RAGLAND D,ADEOLA O.Additivity and associative effects of metabolizable energy and amino acid digestibility in barley and canola meal for White Pekin ducks[J].Poultry Science,2001,80:1600-1606.

[13] HONG D,RAGLAND D,ADEOLA O.Additivity and associative effects of metabolizable energy and amino acid metabolizability of corn,soybean meal and wheat red dog for White Pekin ducks[J].Journal of Animal Science,2002,80:3222-3229.

[14] DALE N M,FULLER H L.Additivity of true metabolizable energy values as measured with roosters,broiler chicks and poults[J].Poultry Science,1980,59:1941-1942.

[15] 霍启光.饲料生物学评定技术[M].北京:中国农业科技出版社,1996.

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