Effects of Different Selenium Sources and Levels on Selenium Content, Antioxidant and Gene Expression in Liver of Breeder Roosters

CHEN Xiuyun; HUA Jing; YANG Zuojun; WANG Xiaoxia; YANG Kailun; ZHANG Jie

doi:10.3969/j.issn.1006-267x.2013.09.027

2013 , Vol. 25 >Issue 9: 2126 - 2135

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.09.027

Short Communications

Effects of Different Selenium Sources and Levels on Selenium Content, Antioxidant and Gene Expression in Liver of Breeder Roosters

CHEN Xiuyun ,
HUA Jing ,
YANG Zuojun ,
WANG Xiaoxia ,
YANG Kailun ,
ZHANG Jie

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1. College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China;
2. College of Animal Science and Technology, Beijing Agricultural University, Beijing 102206, China;
3. Biotechnology College, Beijing Agricultural University, Beijing 102206, China;
4. District Commission of Beijing's Changping District, Beijing 102200, China;
5. Animal Husbandry and Veterinary Department, Beijing Vocational College of Agriculture, Beijing 102442, China

Received date: 2013-03-07

Online published: 2013-08-18

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Abstract

This experiment was conducted to investigate the effects of different selenium sources and levels on selenium content, antioxidant, selenoenzyme activity and glutathione peroxidase 4 (GPx4) gene mRNA relative expression level in liver of breeder roosters. A single factor design was adopted, and one hundred and ninety-six 150-day-old healthy Hy-Line brown breeder roosters were randomly divided into seven groups with four replicates in each group and seven roosters in each replicate. The control group was fed a basal diet, and the experimental groups were fed the diets supplemented with 0.4, 0.8 and 1.2 mg/kg seleno-chitosan (SC) and sodium selenite (SS), respectively. The experiment lasted for 35 days. The results showed as follows: 1) different selenium sources and levels had no significant effect on growth performance (P>0.05). 2) The supplementation of SS and SC significantly increased the selenium content in plasma and liver (P<0.05), and with the dietary selenium level increasing, the body selenium content had increased. 3) Compared with the control group, the activitives of glutathione peroxidase (GSH-Px), total superoxide dismutase and catalase in SC groups were significantly increased (P<0.05); and the malondialdehyde content was significantly decreased (P<0.05). 4) Compared with the control group, the activitives of thioredoxin reductase and the iodothyonine deiodinases Ⅰ in SS and SC groups were significantly increased (P<0.05); and SC groups were significantly higher than SS group (P<0.05). 5) When selenium supplemental level was 0.8 mg/kg, the GPx4 gene mRNA relative expression level in SC group was significantly higher than that in control group (P<0.05). These results show that different selenium sources and levels both influence the selenium content, antioxidant, selenoenzyme activity and GPx4 gene mRNA relative expression level in liver of breeder roosters; the organic selenium is better than inorganic selenium.

Key words： seleno-chitosan; liver; selenium content; antioxidant; breeder roosters

Cite this article

CHEN Xiuyun , HUA Jing , YANG Zuojun , WANG Xiaoxia , YANG Kailun , ZHANG Jie . Effects of Different Selenium Sources and Levels on Selenium Content, Antioxidant and Gene Expression in Liver of Breeder Roosters[J]. Chinese Journal of Animal Nutrition, 2013 , 25(9) : 2126 -2135 . DOI: 10.3969/j.issn.1006-267x.2013.09.027

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