Diversity of Rumen Microflora under Different Cellulose Enrichment Conditions Analyzed by PCR-DGGE

ZENG Yan; SUN Peng; NI Xueqin; YANG Jie; ZENG Dong; ZHANG Hongyu

doi:10.3969/j.issn.1006-267x.2013.09.028

Chinese Journal of Animal Nutrition >

2013 , Vol. 25 >Issue 9: 2136 - 2142

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.09.028

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Diversity of Rumen Microflora under Different Cellulose Enrichment Conditions Analyzed by PCR-DGGE

ZENG Yan ,
SUN Peng ,
NI Xueqin ,
YANG Jie ,
ZENG Dong ,
ZHANG Hongyu

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1. College of Animal Medicine, Sichuan Agricultural University, Ya'an 625014, China;
2. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Ya'an 625014, China

Received date: 2013-03-16

Online published: 2013-08-18

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Abstract

This experiment was conducted to study the diversity of rumen microflora under different cellulose enrichment conditions by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Carboxymethyl cellulose (CMC), peptone-cellulose (PCS), J and K mediums were used in this study, and rumen contents were incubated at 37 and 50℃, respectively. The diversity of rumen mircroflora was analyzed by PCR-DGGE, cloning and sequencing of common and specific bands in DGGE profiles, cluster analysis and principal component analysis. The results showed as follows: there were differences among DGGE profiles of different mediums at 37℃, the similarity coefficient between J and PCS mediums was 0.77, and that between J and CMC mediums was 0.76. The DGGE profiles of different mediums were similar to each other at 50℃, the similarity coefficient between J and PCS mediums was 0.78, and that between CMC and PCS mediums reached 0.84. The common bands were Streptococcus gallolyticus and Ureibacillus themosphaericus,and were predominant in rumen contents; the specific bands were Streptococcus gallolyticus, Pseudomonas mosselii, Alcaligenes and uncultured Lachnospiraceae bacterium clone. In conclusion, the diversity of rumen microflora can be affected by different cellulose enrichment mediums and culture temperatures.

Key words： rumen microflora; cellulose; PCR-DGGE; cluster analysis; principal component analysis

Cite this article

ZENG Yan , SUN Peng , NI Xueqin , YANG Jie , ZENG Dong , ZHANG Hongyu . Diversity of Rumen Microflora under Different Cellulose Enrichment Conditions Analyzed by PCR-DGGE[J]. Chinese Journal of Animal Nutrition, 2013 , 25(9) : 2136 -2142 . DOI: 10.3969/j.issn.1006-267x.2013.09.028

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