Effects of Whole Peptidoglycan from Nocardia seriolae on Non-Specific Immunity of Channa argus

PENG Chengyuan; XIE Jun; JIN Shan; ZHAO Qingsong; CHEN Yin; WANG Guoliang

doi:10.3969/j.issn.1006-267x.2013.09.030

Chinese Journal of Animal Nutrition >

2013 , Vol. 25 >Issue 9: 2150 - 2159

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.09.030

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Effects of Whole Peptidoglycan from Nocardia seriolae on Non-Specific Immunity of Channa argus

PENG Chengyuan ,
XIE Jun ,
JIN Shan ,
ZHAO Qingsong ,
CHEN Yin ,
WANG Guoliang

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School of Marine Sciences, Ningbo University, Ningbo 315211, China

Received date: 2013-03-27

Online published: 2013-08-18

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Abstract

In order to research the immune effects of whole peptidoglycan (WPG) from Nocardia seriolae for fish, the non-specific immune indices such as haemocytes phagocytic index (PI), serum alternative complement pathway activity (ACH50), and superoxide dismutase (SOD), acid phosphatase (ACP), alkaline phosphatase (AKP) and lysozyme (LSZ) activities in different tissues of Channa argus were determined by injection with 0.3 mL/tail 0.1% solution of WPG from N. seriolae intraperitoneally. The C. argus injected 0.3 mL/tail 0.7% sterilized saline as a control. The samples, including serum, mucus, liver and head kidney, were taken from fish on day 0, 1, 2, 3, 4, 5, 6, 7 and 8 post-injection. Moreover, the immune protective efficacy of WPG from N. seriolae against N. seriolae in C. argus was measured by the challenge experiment. The results showed as follows: compared with the control group, the erythrocytes PI, leucocytes PI and serum ACH50 in trial group were significantly increased on day 1 (P<0.05 or P<0.01) and then got the peak on day 2, 2 and 1, which were 2.45%, 3.64% and 306.67 unit/mL, respectively. In serum, the AKP and LSZ activities in trial group were significantly increased from day 4 and 3 (P<0.05 or P<0.01), respectively, and the ACP and SOD activities were all significantly increased from day 1 to day 7 compared with the control group (P<0.05 or P<0.01). After injection WPG from N. seriolae, the activity of ACP in mucus, liver and head kidney was all increased and then got the peak on day 2, 4 and 7, respectively; no significant difference was found in the activity of AKP in mucus and head kidney on any time points (P>0.05), and the activity of AKP in liver was significantly increased on day 3 and 4 (P<0.05 or P<0.01); no significant difference was found in the activity of SOD in liver and head kidney on any time points (P>0.05), while the activity of SOD in mucus was significantly increased on any time points (P<0.05 or P<0.01); the activity of LSZ in mucus and liver was significantly increased on day 1 (P<0.05 or P<0.01) and then got the peak on day 2, while the activity of LSZ in head kidney was firstly increased and then decreased and only got a significant increase on day 5 (P<0.05). N. seriolae challenge experiment result showed that protective rate for C. argus after the WPG intraperitoneal injection on day 7, 21 and 35 were 35%, 55% and 60%, respectively. The above results indicate that WPG from N. seriolae has a significantly regulating effect on haemocytes PI, serum ACH50, and tissue ACP, AKP, SOD and LSZ activities of C. argus, but the strength of regulating effect is different in different tissues. There is a significant protective effect for Nocardia infected C. argus with the WPG from N. seriolae intraperitoneal injection more than 21 days.

Key words： Nocardia seriolae; whole peptidoglycan; Channa argus; non-specific immunity; immune protection

Cite this article

PENG Chengyuan , XIE Jun , JIN Shan , ZHAO Qingsong , CHEN Yin , WANG Guoliang . Effects of Whole Peptidoglycan from Nocardia seriolae on Non-Specific Immunity of Channa argus[J]. Chinese Journal of Animal Nutrition, 2013 , 25(9) : 2150 -2159 . DOI: 10.3969/j.issn.1006-267x.2013.09.030

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