Effects of Relative Humidity and Ammonia Stress on Plasma Ammonia Level and Cytokine Contents of Broilers

WEI Fengxian; HU Xiaofei; ZHANG Minhong; LI Shaoyu; XU Bin; LIN Ping; SUN Quanyou; LI Hao

doi:10.3969/j.issn.1006-267x.2013.10.008

Chinese Journal of Animal Nutrition >

2013 , Vol. 25 >Issue 10: 2246 - 2253

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.10.008

Poultry Nutrition

Effects of Relative Humidity and Ammonia Stress on Plasma Ammonia Level and Cytokine Contents of Broilers

WEI Fengxian ,
HU Xiaofei ,
ZHANG Minhong ,
LI Shaoyu ,
XU Bin ,
LIN Ping ,
SUN Quanyou ,
LI Hao

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1. Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China;
2. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, The Chinese Academy of Agricultural Sciences, Beijing 100193, China;
3. The Henan Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China;
4. Henan Aerial Forest Fire Station, Zhengzhou 450003, China

Received date: 2013-04-23

Online published: 2013-09-16

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Abstract

This experiment was conducted to study the effects of different relative humidity (RH) and ammonia (NH₃) stress in poultry house on plasma ammonia level and cytokine contents of broilers. A total of 288 21-day-old broilers with an average bodyweight were divided into 6 treatments with 6 replicates per treatment and 8 broilers per replicate. The 6 treatments were as follows: Treatment 1, 30 mg/kg NH₃+35% RH; treatment 2, 30 mg/kg NH₃+60% RH, treatment 3, 30 mg/kg NH₃+85% RH; treatment 4, 70 mg/kg NH₃+35% RH; treatment 5, 70 mg/kg NH₃+60% RH; treatment 6, 70 mg/kg NH₃+85% RH. The experiment was conducted in climate chambers, and the experiment lasted for 3 weeks. The results showed as follows: 1) NH₃ concentration had a significant influence on plasma ammonia level (P<0.05), the plasma ammonia level was increased with the increase of time in climate chambers. 2) RH had a significant influence on serum interleukin 1β (IL-1β) content at 3 weeks in climate chambers (P<0.05), the contents of IL-1β and interleukin 6 (IL-6) in serum were increased with the increase of time in climate chambers. 3) NH₃ concentration had a significant influence on serum interleukin 4 (IL-4) content at 1 and 2 weeks in climate chambers (P<0.05), also had a significant influence on serum interleukin 10 (IL-10) content at 1 and 3 weeks in climate chambers (P<0.05). RH had a significant influence on serum IL-10 content at 1 week in climate chambers (P<0.05). It is concluded that RH and NH₃ stress impair the immune function of broilers. With the increase of time in climate chambers, the body obtains certain ability to adapt to the environment, the immunosuppressive effects has a reduced tendency. Moreover, the immune suppression of broilers caused by 70 mg/kg NH₃+85% RH cannot be alleviated.

Key words： relative humidity; NH₃; broilers; plasma ammonia; cytokine

Cite this article

WEI Fengxian , HU Xiaofei , ZHANG Minhong , LI Shaoyu , XU Bin , LIN Ping , SUN Quanyou , LI Hao . Effects of Relative Humidity and Ammonia Stress on Plasma Ammonia Level and Cytokine Contents of Broilers[J]. Chinese Journal of Animal Nutrition, 2013 , 25(10) : 2246 -2253 . DOI: 10.3969/j.issn.1006-267x.2013.10.008

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