Effects of Dietary Metabolizable Energy Level on Carass Performance and Intramuscular Fat Content of Beijing You Chickens

BAI Jie; CHEN Jilan; LI Dongli; YUE Wenbin; ZHENG Maiqing; ZHAO Guiping; WEN Jie

doi:10.3969/j.issn.1006-267x.2013.10.010

2013 , Vol. 25 >Issue 10: 2266 - 2276

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.10.010

Molecular Nutrition

Effects of Dietary Metabolizable Energy Level on Carass Performance and Intramuscular Fat Content of Beijing You Chickens

BAI Jie ,
CHEN Jilan ,
LI Dongli ,
YUE Wenbin ,
ZHENG Maiqing ,
ZHAO Guiping ,
WEN Jie

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1. National Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China

Received date: 2013-04-16

Online published: 2013-09-16

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Abstract

This experiment was conducted to investigate the effects of dietary metabolizable energy (ME) level on carcass performance and intramuscular fat content in pectoral and leg muscles of Beijing You chickens with different genotypes of adipocyte fatty acid-binding protein (A-FABP) gene. Four hundred and eighty 42-day-old male Beijing You chickens were allotted to 4 groups with 6 replicates per group and 20 birds per replicate, and fed with diets containing 11.76, 12.39, 13.02 and 13.65 MJ/kg ME (group Ⅰ to group Ⅳ). All birds were reared in three layer ladder-cages for 48 days. At 90 days of age, all birds were killed and carcass performance was analyzed. Seven birds from one replicate were randomly selected, and 10 mL blood was sampled from wing venous to detect single nucleotide polymorphism of A-FABP gene. There were 3 genotypes including homozygote (MM), heterozygote (MN) and mutation (NN) according to C→T mutation at the 51st base in exon 1 of A-FABP gene. The results showed as follows: 1) the live body weight, dressing percentage, and eviscerated carcass percentage in group Ⅲ were significantly higher than those in groups I and Ⅱ (P<0.05), whereas were similar to those in group Ⅳ (P>0.05). 2) The abdominal fat percentage of Beijing You chickens fed with the diet containing 13.65 MJ/kg ME was 1.65, 2.42 and 2.50 fold of that of birds fed with the diet containing 11.76, 12.39 and 13.02 MJ/kg ME, respectively (P<0.05). The higher ME (13.65 MJ/kg) significantly increased the abdominal fat percentage of Beijing You chickens with NN genotype as compared with the media ME (12.39 and 13.02 MJ/kg) groups (P<0.05), but it had no significant effects on that of birds with MN and MM genotypes (P>0.05). 3) The intramuscular fat (IMF) content of Beijing You chickens fed with the diet containing 12.39 MJ/kg ME was 1.35, 1.34 and 1.32 fold of that of birds fed with the diet containing 11.76, 13.02 and 13.65 MJ/kg ME, respectively (P<0.05), and there was no significant difference among birds fed with the diet containing 11.76, 13.02 and 13.65 MJ/kg ME (P>0.05). The Beijing You chickens with MM genotype had higher IMF content in leg muscle and A-FABP gene mRNA expression level in adipose tissue than those with MN and NN genotypes (P<0.05). The IMF content in leg muscle significantly correlated to the A-FABP gene mRNA expression level and the polymorphism of A-FABP (P<0.01). These results suggest that the diet containing 13.02 MJ/kg ME can increase the live body weight, dressing percentage and eviscerated carcass percentage of Beijing You chickens aged from 7 to 13 weeks. The diet containing higher ME (13.65 MJ/kg) can increase the abdominal fat percentage of Beijing You chickens, especially the chickens with the genotypes of C→T mutation in exon 1 of A-FABP gene, but it does not increase the IMF content in pectoral and leg muscles.

Key words： Beijing You chickens; carcass performance; adipocyte fatty acid-binding protein; gene polymorpholism

Cite this article

BAI Jie , CHEN Jilan , LI Dongli , YUE Wenbin , ZHENG Maiqing , ZHAO Guiping , WEN Jie . Effects of Dietary Metabolizable Energy Level on Carass Performance and Intramuscular Fat Content of Beijing You Chickens[J]. Chinese Journal of Animal Nutrition, 2013 , 25(10) : 2266 -2276 . DOI: 10.3969/j.issn.1006-267x.2013.10.010

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