A Study on Relationship between Dietary NSC/NDF and Methane Production of High Concentrate Diets using in Vitro Incubation Technique

ZHENG Wensi; ZHAO Guangyong; ZHANG Tingting; NIU Wenjing; DONG Ruilan

doi:10.3969/j.issn.1006-267x.2013.10.015

2013 , Vol. 25 >Issue 10: 2315 - 2324

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.10.015

Ruminant Nutrition

A Study on Relationship between Dietary NSC/NDF and Methane Production of High Concentrate Diets using in Vitro Incubation Technique

ZHENG Wensi ,
ZHAO Guangyong ,
ZHANG Tingting ,
NIU Wenjing ,
DONG Ruilan

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State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China

Received date: 2013-03-27

Online published: 2013-09-16

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Abstract

This experiment was conducted to study the relationship between dietary non-structural carbohydrates/neutral detergent fiber (NSC/NDF) and methane (CH₄), production of high concentrate diets using in vitro incubation technique. Fifty diets with 5 ratios of concentrate to roughage (60:40, 70:30, 80:20, 90:10 and 100:0) and 10 diets for each ratio were formulated. An in vitro gas test was used for 48 h incubation. After that, gas and liquid samples were collected for the determination of CH₄ and CO₂ production in gas and volatile fatty acids (VFA) production in liquid. The results showed that significantly positive relationships were found between dietary NSC/NDF and CH₄, CO₂ and total VFA (TVFA) production (P<0.01), and significantly negative relationships were found between dietary NSC/NDF, acetate/propionate and CH₄/TVFA (P<0.01), but regression coefficients were all low. It is concluded that in vitro relative CH₄ production of high concentrate diet is decreased with the increase of dietary NSC/NDF, but the low regression coefficients indicate that in vitro CH₄ production can not be accurately predicted merely based on dietary NSC/NDF.

Key words： NSC/NDF; in vitro incubation; methane; volatile fatty acids

Cite this article

ZHENG Wensi , ZHAO Guangyong , ZHANG Tingting , NIU Wenjing , DONG Ruilan . A Study on Relationship between Dietary NSC/NDF and Methane Production of High Concentrate Diets using in Vitro Incubation Technique[J]. Chinese Journal of Animal Nutrition, 2013 , 25(10) : 2315 -2324 . DOI: 10.3969/j.issn.1006-267x.2013.10.015

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