Effects of Bactrian Camel Milk on Hepatocyte Apoptosis of Type 2 Diabetes Mellitus Rats

WANG Xueying; ZHANG Quanwei; ZHANG Yong; ZHANG Quanlong; JI Peizhong; LI Yalan; ZHAO Xingxu

doi:10.3969/j.issn.1006-267x.2013.10.026

Chinese Journal of Animal Nutrition >

2013 , Vol. 25 >Issue 10: 2422 - 2429

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.10.026

Feed Science and Technology

Effects of Bactrian Camel Milk on Hepatocyte Apoptosis of Type 2 Diabetes Mellitus Rats

WANG Xueying ,
ZHANG Quanwei ,
ZHANG Yong ,
ZHANG Quanlong ,
JI Peizhong ,
LI Yalan ,
ZHAO Xingxu

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1. College of Veterinary Medicine, Gansu Agriculture University, Lanzhou 730070, China;
2. Hospital of Medicine Branch, Lanzhou Military Region Hospital, Lanzhou 730040, China

Received date: 2013-04-26

Online published: 2013-09-16

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Abstract

This study was conducted to investigate the protective effects of Bactrian camel milk on hepatocyte apoptosis of type 2 diabetes mellitus (T2DM) rats. Sixty adult male rats with similar month of age were randomly divided into five groups, which were negative control (C) group (healthy rats, basal diet), diabetes model control (DMC) group (T2DM rats), low concentration camel milk (LCM) group (2 mL/d, T2DM rats), high concentration camel milk (HCM) group (5 mL/d, T2DM rats) and metformin hydrochloride (MTH) group (200 mg/kg, T2DM rats), respectively. The experiment lasted for 4 weeks. The results showed as follows: compared with C and DMC groups, the blood glucose concentration in MTH and HCM groups was significantly decreased (P<0.01). The hepatocyte was swollen, partial nuclear fragmentation, shrinkage and dissolution in DMC group, while the morphology was normal, and the nuclear was clear in LCM, HCM and MTH groups. Compared with DMC group, the synthesis of B cell lymphoma/lewkmia-2 (BCL-2) was increased in HCM and MTH groups, while that in BCL-2-associated X protein (BAX) was decreased in HCM and MTH groups. The relative expression level of BCL-2 mRNA was the highest in the MTH group, while the relative expression level of BAX mRNA reached its peak in LCM and MTH groups. The relative expression level of BCL-2 mRNA was the highest in MTH group, and that of BAX was higher in LCM and HCM groups than that in the other two groups. BCL-2 and BAX proteins could be detected in DMC, LCM, HCM and MTH groups. The results indicate that Bactrian milk can suppress cell apoptosis and promote cell proliferation through inhibiting BAX protein synthesis, but encouraging BCL-2 protein synthesis, thus reduce the hepatocyte injury and decrease the blood glucose concentration of T2DM rats, which provides a new adjunctive treatment for diabetes mellitus.

Key words： camel milk; type 2 diabetes; hepatocyte; apoptosis

Cite this article

WANG Xueying , ZHANG Quanwei , ZHANG Yong , ZHANG Quanlong , JI Peizhong , LI Yalan , ZHAO Xingxu . Effects of Bactrian Camel Milk on Hepatocyte Apoptosis of Type 2 Diabetes Mellitus Rats[J]. Chinese Journal of Animal Nutrition, 2013 , 25(10) : 2422 -2429 . DOI: 10.3969/j.issn.1006-267x.2013.10.026

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