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Intestinal Microflora Analysis of Pre-and Post-Weaning Piglets Using by 454 Pyrosequencing Technology

  • WANG Yibing ,
  • ZHANG Xiaoping ,
  • HUANG Yi ,
  • LI Weifen
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  • 1. Institute of Feed Science, College of Animal Science, Zhejiang University, Hangzhou 310058, China;
    2. College of Animal Science and Technology, Guangxi University, Nanning 530004, China

Received date: 2013-04-11

  Online published: 2013-09-16

Abstract

This present study was designed to analyze the intestinal microflora change of pre-and post-weaning piglets using by 454 pyrosequencing technology. Twelve newborn piglets (Duroc×Landrace×Large White) with the similar parity, birth time, and body weight were used in this experiment. All the experimental piglets were conventionally suckled by sows until weaning (25 days of age). The diet of sows did not contain antibiotics, and all piglets had free access to pre-starter diets from 12 days of age. Three piglets were randomly selected and killed at 25 (pre-weaning) and 32 (post-weaning) days of age, and cecal contents were collected. Microflora structure of cecal contents of pre-and post-weaning piglets were evaluated using by 454 pyrosequencing technology. The results showed that compared with the pre-weaning piglets, the diarrhea rate of post-weaning piglets was significantly increased (P<0.05); the diversity of intestinal microflora was increased; no difference was observed in the content of Bacteroides of post-weaning piglets (P>0.05); the content of Firmicutes was significantly increased by 63.95% (P<0.05), while the contents of Fusobacteria and Proteobacteria were significantly decreased by 100.00% and 70.54% (P<0.05). For Firmicutes, a further analysis was made. At class level, the contents of Bacilli and Negativicutes of post-weaning piglets were decreased (P>0.05), while the contents of Negativicutes, Erysipelotrichia and unclassified Firmicutes were increased (P>0.05). The relative abundance of each species from phylum to genus was different between pre-and post-weaning piglets. Some genera (such as Bilophila, Fusobacteriu and Flavobacteriaceae, etc.) only appeared in the pre-weaning piglets, while some other genera(such as Facklami and Sarcina, etc.)existed only in the post-weaning piglets. In conclusion, the diversity and the composition of intestinal microflora are altered in post-weaning piglets.

Cite this article

WANG Yibing , ZHANG Xiaoping , HUANG Yi , LI Weifen . Intestinal Microflora Analysis of Pre-and Post-Weaning Piglets Using by 454 Pyrosequencing Technology[J]. Chinese Journal of Animal Nutrition, 2013 , 25(10) : 2440 -2446 . DOI: 10.3969/j.issn.1006-267x.2013.10.028

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